TSC1 enables plastid development under dark conditions, contributing to rice adaptation to transplantation shock
TSC1 enables plastid development under dark conditions, contributing to rice adaptation to transplantation shock
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TSC1 使质体在黑暗条件下发育，有助于水稻适应移植休克
DOI：
10.1111/jipb.12621
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发表时间：
2018-02-01
影响因子：
11.4
通讯作者：
Teng, Sheng
中科院分区：
文献类型：
--
作者：
Shi, Xiaoliang;Chen, Sunlu;Teng, Sheng
自从几千年前从野生稻驯化以来，水稻主要通过移植来栽培。水稻秧苗在从苗圃移栽到稻田的过程中，会经历移栽休克，影响其生理和产量。然而，移植休克和水稻适应这种冲击的机制在很大程度上是未知的。在这里，我们分离出一个对移植敏感的叶绿体缺陷（tsc1）水稻突变体，该突变体在移植后产生白化病叶。阻断光到达幼叶和叶原基导致移栽tsc 1幼苗叶绿体缺陷。TSC 1编码与AtNAP 14同源的非经典三磷酸腺苷结合盒（ABC）转运蛋白，并且是蓝藻来源的。我们证明，TSC 1控制水稻质体发育在黑暗条件下，和功能独立的光信号。然而，光拯救了tsc1突变表型的光谱独立的方式。TSC 1上调移植后，和调制的铁和铜的水平，从而调节prolaminate体形成在早期P4阶段的叶发育。因此，TSC 1在缺乏光的情况下对于质体发育是不可或缺的，并且有助于适应移植休克。我们的研究提供了深入了解质体发育的调节，并建立了一个框架，提高水稻移植休克的恢复。
Since its domestication from wild rice thousands of years ago, rice has been cultivated largely through transplantation. During transplantation from the nursery to the paddy field, rice seedlings experience transplantation shock which affects their physiology and production. However, the mechanisms underlying transplantation shock and rice adaptation to this shock are largely unknown. Here, we isolated a transplant-sensitive chloroplast-deficient (tsc1) rice mutant that produces albino leaves after transplantation. Blocking light from reaching the juvenile leaves and leaf primordia caused chloroplast deficiencies in transplanted tsc1 seedlings. TSC1 encodes a noncanonical adenosine triphosphate-binding cassette (ABC) transporter homologous to AtNAP14 and is of cyanobacterial origin. We demonstrate that TSC1 controls plastid development in rice under dark conditions, and functions independently of light signaling. However, light rescued the tsc1 mutant phenotype in a spectrum-independent manner. TSC1 was upregulated following transplantation, and modulated the iron and copper levels, thereby regulating prolamellar body formation during the early P4 stage of leaf development. Therefore, TSC1 is indispensable for plastid development in the absence of light, and contributes to adaptation to transplantation shock. Our study provides insight into the regulation of plastid development and establishes a framework for improving recovery from transplantation shock in rice.