EXPRESSION OF DONOR CLASS-I MAJOR HISTOCOMPATIBILITY ANTIGENS ON THE VASCULAR ENDOTHELIUM OF MOUSE SKIN ALLOGRAFTS
EXPRESSION OF DONOR CLASS-I MAJOR HISTOCOMPATIBILITY ANTIGENS ON THE VASCULAR ENDOTHELIUM OF MOUSE SKIN ALLOGRAFTS
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DOI：
10.1097/00007890-198608000-00015
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发表时间：
1986-08-01
期刊：
影响因子：
6.2
通讯作者：
KOENE, RAP
中科院分区：
文献类型：
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作者：
DEWAAL, RMW;BOGMAN, MJJT;KOENE, RAP
I类MHC抗原在小鼠皮肤同种异体移植物血管内皮上的表达通过静脉注射到受体中后移植物中的放射性标记的单克隆抗I类抗体的体内摄取来评估。通过使用因子VIII相关抗原作为内皮细胞的标记物的双标记免疫荧光显微术证明结合抗体的内皮定位。接受环孢素治疗的患者伴随着移植物中I类抗原表达的低水平，并且在完全没有排斥反应的情况下，在裸受体携带的移植物中测量到类似的低水平。免疫抑制剂治疗后，供体皮肤中I类抗原表达增加。在接受第一次排异反应的皮肤移植物中也观察到增加。我们的结论是，在体内的小鼠皮肤移植物的毛细血管内皮细胞上的I类抗原的表达是可变的，是受者的免疫状态的影响下。
The expression of a class I MHC antigen on the vascular endothelium of mouse skin allografts was assessed by in vivo uptake of radiolabeled monoclonal anti—class-I antibody in the grafts after iv injection into the recipients. Endothelial localization of the bound antibodies was demonstrated via double-labeling immunofluorescence microscopy using factor-VIII-related antigen as a marker for endothelial cells. Treatment of recipients with cyclosporine was accompanied by low levels of class I antigen expression in the grafts, and similarly low levels were measured in grafts carried by nude recipients in the complete absence of rejection. Withdrawal of immunosuppressive therapy was followed by an increased class I an6tigen expression in the donor skin. An increase was also observed in skin grafts undergoing first-set rejection. We conclude that the expression of class I antigens on the capillary endothelium of mouse skin allografts in vivo is variable and is under influence of the immune status of the recipient.