Studies of mitochondrial morphology and DNA amount in the rice egg cell
Studies of mitochondrial morphology and DNA amount in the rice egg cell
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DOI：
10.1007/s00294-009-0277-3
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发表时间：
2010-02
期刊：
影响因子：
2.5
通讯作者：
H. Takanashi;Takayuki Ohnishi;Mirai Mogi;T. Okamoto;S. Arimura;N. Tsutsumi
中科院分区：
文献类型：
--
作者：
H. Takanashi;Takayuki Ohnishi;Mirai Mogi;T. Okamoto;S. Arimura;N. Tsutsumi
在植物营养细胞中，线粒体通常很小，呈颗粒状。与此相反，形状异常的巨型线粒体（大杯形或长杆形）出现在大芽甘蓝、玉米、空心菜和欧洲蕨的卵细胞中。为了表征水稻卵细胞线粒体的特征，我们采用非酶手工解剖法分离水稻未受精卵细胞，并同时观察卵细胞线粒体和线粒体DNA（mtDNA）。这些观察结果表明，水稻卵细胞中的线粒体较小，呈颗粒状，与玉米、玉米、I. nil和bracken。用MitoTracker和SYBR绿色I对线粒体和mtDNA进行双重染色，结果表明，水稻卵细胞线粒体中含有大量的mtDNA。我们还利用实时荧光定量PCR分析了水稻卵细胞中mtDNA的含量。我们定量了每个水稻卵细胞和水稻叶片原生质体中四个线粒体基因的拷贝数。实时荧光定量PCR分析表明，与叶原生质体相比，卵细胞线粒体基因组中编码的所有四个基因的拷贝数都高出十倍以上。
In plant vegetative cells, mitochondria are usually small and grain-shaped. In contrast, unusually shaped giant mitochondria (large cup-shaped or long stretched-rod-shaped) appear in the egg cells of geranium, maize,Ipomoea nil, and bracken. In this study, to characterize egg cell mitochondria in rice, we used nonenzymatic manual dissection to isolate unfertilized egg cells of rice and observed the egg cell mitochondria and mitochondrial DNA (mtDNA) simultaneously. These observations showed that the mitochondria in the rice egg cell are small and grain-shaped, unlike the mitochondria in geranium, maize,I. nil, and bracken. Double staining of mitochondria by MitoTracker and mtDNA by SYBR Green I showed that mitochondria in the rice egg cell have a large amount of mtDNA compared with the rice root protoplast. We also used real-time PCR analysis to quantify the mtDNA amount in the rice egg cell. We quantified the copy numbers of four mitochondrial genes per single rice egg cell and rice leaf protoplast. Real-time PCR analysis revealed that the egg cell has more than ten times more copy numbers of all of four genes encoded in the mitochondrial genome compared with the leaf protoplast.