Levels of lactoferrin, secretory IgA and serum albumin in the tear film of people with keratoconus
Levels of lactoferrin, secretory IgA and serum albumin in the tear film of people with keratoconus
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DOI：
10.1016/j.exer.2011.12.010
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发表时间：
2012-03-01
影响因子：
3.4
通讯作者：
Willcox, Mark Duncan Perry
中科院分区：
文献类型：
--
作者：
Balasubramanian, Sivaraman Arumugam;Pye, David Cecil;Willcox, Mark Duncan Perry
圆锥角膜是一种眼部退化性疾病，会导致角膜形状不规则，从而导致视力严重受损。圆锥角膜的泪液蛋白质组学多年来一直是大量讨论和猜测的话题。本研究旨在检测圆锥角膜患者泪膜中总蛋白、乳铁蛋白、分泌型 IgA 和血清白蛋白的水平。使用毛细管收集基底泪液，并使用地形图仪绘制角膜曲率图。估计泪液中的总蛋白质。使用特异性 ELISA 测量调节蛋白乳铁蛋白、组成蛋白 sIgA 和血清蛋白白蛋白的量。泪液中蛋白质浓度的变化与角膜非球面程度相关。有两倍（p
Keratoconus is a degenerating disease of the eye which causes an irregularly shaped cornea leading to severe impairment of vision. Tear proteomics in keratoconus has been a topic of substantial discussion and speculation over many years. This study was designed to examine the levels of total protein, lactoferrin, secretory IgA and serum albumin in the tear film of people with keratoconus. Basal tears were collected using a capillary tube and corneal curvature was mapped using a topographer. Total protein in tears was estimated. The amount of regulated protein lactoferrin, constitutive protein sIgA and serum protein albumin was measured using specific ELISAs. The changes in protein concentrations in tears were correlated to the degree of corneal asphericity. There was a two-fold (p