Comparison of a two-stage system with low pressure carbon dioxide microbubbles and heat treatment on the inactivation of Saccharomyces pastorianus cells
Comparison of a two-stage system with low pressure carbon dioxide microbubbles and heat treatment on the inactivation of Saccharomyces pastorianus cells
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DOI：
10.1016/j.foodcont.2014.04.034
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发表时间：
2014-12
期刊：
影响因子：
6
通讯作者：
F. Kobayashi;M. Sugiura;H. Ikeura;M. Sato;S. Odake;M. Tamaki
中科院分区：
文献类型：
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作者：
F. Kobayashi;M. Sugiura;H. Ikeura;M. Sato;S. Odake;M. Tamaki
澄清微泡二氧化碳（MB-CO2）与这些混合后加热和加压的两级系统如何影响酵母细胞。低温低压牛乳悬浮液（两级MB-CO2），S。电镜观察巴氏酵母细胞，并用碘化丙啶（PI）染色。此外，从处理中泄漏的核酸和蛋白质的数量。测定巴氏酵母细胞，测定细胞内酶活性。通过扫描电镜观察到皱褶的形状。经加热盘管加热50℃（MB50）和50℃和80℃（H50和H80）热处理的两段mb - co2处理的pastorianus细胞比未处理的（NT）细胞多。透射电镜观察表明，MB50对细胞内底物有直接作用，但对细胞膜影响不大，而H80细胞对细胞膜有明显的损伤。然而，我们认识到MB50细胞的PI强度大于NT、H50和H80细胞，并且H80细胞的核酸和蛋白质泄漏量明显高于NT、MB50和H50细胞。此外，MB50细胞的酶失活效率与H80细胞相同。这些结果估计s。两阶段mb - co2是由于mb - co2作用于细胞膜和细胞内底物，如酶失活。
To clarify how Saccharomyces pastorianus cells were affected by a two-stage system that was heating and pressurizing after microbubbled carbon dioxide (MB-CO2) was mixed with theS. pastorianussuspension at low temperature and pressure (two-stage MB-CO2),S. pastorianuscells were observed by electronic microscopy and stained with propidium iodide (PI). Furthermore, the amounts of nucleic acid and protein leaked from treatedS. pastorianuscells were determined and intracellular enzyme activities were measured. It was observed by scanning electric microscopy that wrinkles inS. pastorianuscells treated by two-stage MB-CO2with a heating coil at 50 °C (MB50) and heat treatments at 50 °C and 80 °C (H50 and H80) were more than those in untreated (NT) cells. Upon observation with transmission electron microscopy, it suggested that MB50 had a direct effect on the intracellular substrate, although little influence on the membrane, whereas H80 cells showed visible damage to cell membranes. However, it was recognized that PI intensity in MB50 cells was great than that in NT, H50 and H80 cells, and that the amount of nucleic acid and protein leaked from H80 cells was significantly higher than that of NT, MB50 and H50 cells. Furthermore, the enzyme inactivation efficiency in MB50 cells was the same as in H80 cells. These results estimate that inactivation ofS. pastorianusby two-stage MB-CO2was due to actions of MB-CO2on the cell membrane and the intracellular substrate such as enzyme inactivation.