Disruption of the MNN10 gene enhances protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae
Disruption of the MNN10 gene enhances protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae
复制标题
DOI：
10.1016/j.femsyr.2004.03.001
复制
发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
Sasnauskas, K
中科院分区：
文献类型：
--
作者：
Bartkeviciute, D;Sasnauskas, K
从乳酸克鲁维酵母超分泌突变株中筛选影响超分泌表型的基因，分离到一个与酿酒酵母MNN 10具有高度同源性的基因KlMNN 10。乳酸克鲁维酵母中KlMNN 10以及酿酒酵母中MNN 10和MNN 11的破坏赋予了超分泌表型。从酿酒酵母分离的MNN 10抑制乳酸克鲁维酵母klmnn 10中的超分泌表型，同源KlMNN 10也是如此。酿酒酵母的基因MNN 10和MNN 11编码负责甘露糖基转移酶复合物的大部分α-1，6-聚合活性的甘露糖基转移酶。这些数据与酵母细胞壁中糖蛋白的结构强烈影响培养基中同源和异源蛋白质的释放的观点一致。该基因组即过度分泌表型的抑制子，对于进一步分析基因功能、过度分泌机制和构建优化用于异源蛋白分泌的新菌株可能是有吸引力的。KlMNNlO具有EMBL登录号AJ 575132。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of MNN10 and MNN11 in Saccharomyces cerevisiae, conferred the super-secreting phenotype. MNN10 isolated from Saccharomyces cerevisiae suppressed the super-secretion phenotype in Kluyveromyces lactis klmnn10, as did the homologous KlMNN10. The genes MNN10 and MNN11 of Saccharomyces cerevisiae encode mannosyltransferases responsible for the majority of the alpha-1,6-polymerizing activity of the mannosyltransferase complex. These data agree with the view that the structure of glycoproteins in a yeast cell wall strongly influences the release of homologous and heterologous proteins in the medium. The set of genes namely the suppressors of the over-secreting phenotype, could be attractive for further analysis of gene functions, over-secreting mechanisms and for construction of new strains optimized for heterologous protein secretion.KlMNN10 has EMBL accession no. AJ575132. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.