Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering
Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering
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DOI：
10.1016/s0142-9612(02)00176-x
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发表时间：
2002-11-01
期刊：
影响因子：
14
通讯作者：
Anseth, KS
中科院分区：
文献类型：
--
作者：
Burdick, JA;Anseth, KS
研究聚乙二醇（PEG）水凝胶作为成骨细胞的封装基质，以评估其在促进骨组织工程方面的适用性。用粘附性精氨酸-甘氨酸-天冬氨酸（RGD）肽序列修饰非粘附性水凝胶，以促进大鼠颅骨成骨细胞的粘附、扩散，从而促进细胞骨架组织。当附着到水凝胶表面时，修饰和未修饰的水凝胶之间附着的成骨细胞的密度和面积显着不同。观察到 RGD 组的浓度依赖性，随着 RGD 浓度的增加，成骨细胞附着和扩散增加，并且仅在最高肽密度的情况下观察到细胞骨架组织。当用 10% 大分子单体形成凝胶时，大多数成骨细胞在光封装过程中存活下来，但当大分子单体浓度分别增加到 20 和 30wt% 时，体外培养第 1 天后，观察到成骨细胞活力下降了约 25% 和 38%。当肽添加到网络中时，细胞活力没有统计学差异。最后，体外培养 4 周后，所有水凝胶中都出现了矿物质沉积，但在整个网络中引入粘附肽后，观察到矿化显着增加。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Poly(ethylene glycol) (PEG) hydrogels were investigated as encapsulation matrices for osteoblasts to assess their applicability in promoting bone tissue engineering. Non-adhesive hydrogels were modified with adhesive Arg-Gly-Asp (RGD) peptide sequences to facilitate the adhesion, spreading, and, consequently, cytoskeletal organization of rat calvarial osteoblasts. When attached to hydrogel surfaces, the density and area of osteoblasts attached were dramatically different between modified and unmodified hydrogels. A concentration dependence of RGD groups was observed, with increased osteoblast attachment and spreading with higher RGD concentrations, and cytoskeleton organization was seen with only the highest peptide density. A majority of the osteoblasts survived the photoencapsulation process when gels were formed with 10% macromer, but a decrease in osteoblast viability of similar to25% and 38% was seen after I day of in vitro culture when the macromer concentration was increased to 20 and 30wt%, respectively. There was no statistical difference in cell viability when peptides were added to the network. Finally, mineral deposits were seen in all hydrogels after 4 weeks of in vitro culture, but a significant increase in mineralization was observed upon introduction of adhesive peptides throughout the network. (C) 2002 Elsevier Science Ltd. All rights reserved.