Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linking
Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linking
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DOI：
10.1128/jvi.76.19.10050-10055.2002
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发表时间：
2002-10-01
影响因子：
5.4
通讯作者：
Rein, A
中科院分区：
文献类型：
--
作者：
Campbell, S;Oshima, M;Rein, A
我们以前报道过，如果小鼠白血病病毒颗粒在轻度氧化剂二硫代苯甲酰胺-2存在下产生，它们不能经历正常的病毒成熟过程。我们现在表明，用还原剂（二硫苏糖醇）处理这些未成熟的颗粒可以诱导它们在体外成熟，这可以通过Gag、Gag- pol和Env蛋白的蛋白水解裂解及其形态来证明。这些颗粒中部分裂解产物的鉴定表明在这些条件下裂解发生的顺序。这可能是一个有用的实验系统，进一步分析逆转录病毒成熟在体外控制条件下。
We previously reported that if murine leukemia virus particles are produced in the presence of the mild oxidizing agent disulfide-substituted benzamide-2, they fail to undergo the normal process of virus maturation. We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol, and Env proteins and by their morphology. The identification of partial cleavage products in these particles suggests the sequence with which the cleavages occur under these conditions. This may be a useful experimental system for further analysis of retroviral maturation under controlled conditions in vitro.