Bioinformatics approach toward identification of candidate genes for zinc and iron transporters in maize
Bioinformatics approach toward identification of candidate genes for zinc and iron transporters in maize
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作者：
R. Chauhan
农作物的生物强化需要确定与微量营养素积累有关的候选基因。对现有的玉米基因组序列进行扫描，鉴定出33个基因，这些基因被预测与玉米的铁和锌运输有关。YS家族15个，ZIP家族9个，Nramp家族6个，铁蛋白家族2个，Fro家族1个。每个基因家族的成员都有功能特征基因的特征序列和跨膜结构域。类似于水稻命名系统的基因已经被命名，例如ZmZIP代表Zea Mays ZIP类蛋白，而ZmYS基因、ZmNramp基因等代表其他基因家族成员。在候选基因中发现了简单序列重复和单核苷酸多态。本研究从已有的基因组序列数据中为玉米锌、铁运输的候选基因提供了有用的资源。候选基因有望在玉米的遗传定位、关联定位、分子标记辅助选择和转基因植株的培育等方面发挥重要作用。
Biofortification of crop plants requires the identification of candidate genes involved in micronutrient accumulation. Scanning of available maize genome sequence resulted in the identification of 33 genes predicted to be involved in iron and zinc transport in maize. Fifteen genes belong to the YS family, 9 to ZIP family, six to Nramp family, two to ferritin family and one to FRO family. Members of each gene family possessed characteristic signature sequences and transmembrane domains of functionally characterized genes. Genes have been named analogous to a rice nomenclature system such as ZmZIP for Zea mays ZIP-like proteins and similarly ZmYS genes, ZmNramp genes, etc. for other gene family members. Simple sequence repeats as well as single nucleotide polymorphisms were identified in the candidate genes. The current study has provided a useful resource of candidate genes for zinc and iron transport in maize from the available genome sequence data. Candidate genes are expected to be of potential use in genetic and association mapping, molecular marker-assisted selection and development of transgenic plants for micronutrient enrichment traits in maize.