A rapid technique for screening of lovastatin-producing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay
A rapid technique for screening of lovastatin-producing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay
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DOI：
10.1016/s0167-7012(99)00135-9
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发表时间：
2000-03-01
影响因子：
2.2
通讯作者：
Sadhukhan, AK
中科院分区：
文献类型：
--
作者：
Kumar, MS;Kumar, PM;Sadhukhan, AK
菌株改良计划的成功取决于诱变处理后可以筛选的分离株的数量。建立了一种快速筛选高产菌株的技术。利用洛伐他汀的抗真菌特性来产生对粗糙脉孢菌的抑制区的"琼脂塞"方法不仅经济而且劳动强度更低。我们能够分离到一个高产菌株，其生产能力提高了138%，相比，在深层发酵过程中的亲株。(C)2000 Elsevier Science BN。All rights reserved.
The success of strain improvement programme depends on the number of isolates that can be screened after mutagenic treatment. A technique to rapidly screen large number of high-yielding isolates was developed. The 'agar plug' method that utilizes the anti-fungal property of lovastatin to produce a zone of inhibition against Neurospora crassa was not only economical but also less labour-intensive. We were able to isolate a high-yielding strain, the productivity of which increased by 138% as compared to the parent strain in the submerged fermentation process. (C) 2000 Elsevier Science BN. All rights reserved.