A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.
A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.
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DOI：
10.1002/jms.3827
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Sweedler JV
中科院分区：
文献类型：
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作者：
Li B;Comi TJ;Si T;Dunham SJ;Sweedler JV
琼脂平板上培养的生物膜的MALDI成像是具有挑战性的，因为与基质沉积到琼脂上有关的问题。我们描述了2，5-二羟基苯甲酸溶液的一步喷雾应用，用于琼脂上水合枯草杆菌生物膜的直接MALDI成像。使用优化的喷枪和自制的自动喷雾器，从B可视化信号代谢物和同类相食因子的区域特异性分布。在生物膜促进培养基上培养的枯草杆菌细胞。该方法在水合样品上提供均匀、相对干燥的涂层，与筛分基质应用相比，提高了点到点信号的可重复性，并且易于适用于对一系列琼脂基生物膜进行成像。
MALDI imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct MALDI imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared to sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms.