Toward rapid infectious disease diagnosis with advances in surface-enhanced Raman spectroscopy
Toward rapid infectious disease diagnosis with advances in surface-enhanced Raman spectroscopy
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DOI：
10.1063/1.5142767
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发表时间：
2020-06-28
影响因子：
4.4
通讯作者：
Dionne, Jennifer
中科院分区：
文献类型：
--
作者：
Tadesse, Loza F.;Safir, Fareeha;Dionne, Jennifer
在大流行时代，快速传染病诊断至关重要。表面增强拉曼光谱（Sers）有望实现灵敏和特异的诊断，包括快速的即时检测和药物敏感性测试。Sers利用入射光子与分子振动相互作用产生的非弹性光散射，通过共振金属或介电纳米结构增强了几个数量级。虽然Sers提供了样品的光谱指纹，但由于光谱增强的一致性、光谱解释的复杂性、特异性和灵敏度不足以及从患者样品收集到光谱采集的低效工作流程方面的挑战，临床翻译滞后。在这里，我们强调了最近的补充进展，解决这些缺点，包括（1）设计无标记的Sers基板和数据处理算法，提高光谱信号和可解释性，为广泛的病原体筛选分析必不可少的;（2）开发新的捕获和亲和剂，如适配体和聚合物，确定特定病原体的存在或不存在的关键;以及（3）用于有效临床样品处理的微流体和生物打印平台。我们还描述了低成本，即时护理，光学Sers硬件的发展。我们的论文重点关注Sers用于病毒和细菌检测，希望加速传染病诊断，监测和疫苗开发。随着Sers基底、机器学习、微流体和生物打印技术的进步，Sers的特异性、灵敏度和速度可以很容易地从实验室工作台转移到患者床边，加速护理点诊断、个性化医疗和精准健康。
In a pandemic era, rapid infectious disease diagnosis is essential. Surface-enhanced Raman spectroscopy (SERS) promises sensitive and specific diagnosis including rapid point-of-care detection and drug susceptibility testing. SERS utilizes inelastic light scattering arising from the interaction of incident photons with molecular vibrations, enhanced by orders of magnitude with resonant metallic or dielectric nanostructures. While SERS provides a spectral fingerprint of the sample, clinical translation is lagged due to challenges in consistency of spectral enhancement, complexity in spectral interpretation, insufficient specificity and sensitivity, and inefficient workflow from patient sample collection to spectral acquisition. Here, we highlight the recent, complementary advances that address these shortcomings, including (1) design of label-free SERS substrates and data processing algorithms that improve spectral signal and interpretability, essential for broad pathogen screening assays; (2) development of new capture and affinity agents, such as aptamers and polymers, critical for determining the presence or absence of particular pathogens; and (3) microfluidic and bioprinting platforms for efficient clinical sample processing. We also describe the development of low-cost, point-of-care, optical SERS hardware. Our paper focuses on SERS for viral and bacterial detection, in hopes of accelerating infectious disease diagnosis, monitoring, and vaccine development. With advances in SERS substrates, machine learning, and microfluidics and bioprinting, the specificity, sensitivity, and speed of SERS can be readily translated from laboratory bench to patient bedside, accelerating point-of-care diagnosis, personalized medicine, and precision health.