Linkage map of nine loci defined by polymorphic DNA markers assigned to rat chromosome 13.
Linkage map of nine loci defined by polymorphic DNA markers assigned to rat chromosome 13.
复制标题
由分配给大鼠 13 号染色体的多态性 DNA 标记定义的 9 个基因座的连锁图。
作者：
E. Remmers;E. Goldmuntz;H. Zha;P. Mathern;Y. Du;L. Crofford;R. Wilder
利用F344/N大鼠和LEW/N大鼠的单杂交，建立了由多态性DNA标记定义的9个位点的遗传图谱。该标记包含5个基因的多态性简单序列重复序列，分别是肾素（Ren）、心肌肌钙蛋白T （Tnnt3）、synaptotagmin （Syt2）、Na+、K(+)- atp酶催化亚基（Atp1a2）、Asp-、Gly-、Glu-和Leu-tRNA基因簇（Trnegl），以及4个匿名DNA片段。对F344/N和LEW/N大鼠F2杂交后代中这些标记的等位基因分离分析显示，位点顺序和对间距离为：d13n1 -1 cm - ren1 cM-Tntt3-0 cM-Syt2-12 cM-D13N2-25 cM-Atp1a2-0 cm - trln -7 cM-D13N3-4 cM-D13N4。其中三个基因座，Ren， Trnegl和Atp1a2，先前被分配到大鼠13号染色体上。除Ren外，其他位点此前均未通过连锁分析绘制。这些位点的标记在13个自交系（F344/N、LEW/N、LOU/MN、WBB1/N、WBB2/N、MR/N、MNR/N、ACI/N、SHR/N、WKY/N、BN/SsN、BUF/N和LER/N）中检测到高度多态性，每个标记检测到2 ~ 8个等位基因。这些标记扩大了大鼠的遗传图谱，应该是未来遗传研究的有价值的工具。对所有分配给大鼠13号染色体的位点的人类和小鼠比较图谱信息的检查显示，与人类1号染色体的q臂和小鼠1号染色体的远端部分有显著的同源性守恒。
A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the Asp-, Gly-, Glu-, and Leu-tRNA gene cluster (Trnegl), as well as four anonymous DNA segments. Analysis of the segregation of the alleles of these markers in F2 intercross progeny of F344/N and LEW/N rats indicated the following locus order and distances between pairs of loci: D13N1-5 cM-Ren-1 cM-Tntt3-0 cM-Syt2-12 cM-D13N2-25 cM-Atp1a2-0 cM-Trnegl-7 cM-D13N3-4 cM-D13N4. Three of the loci, Ren, Trnegl, and Atp1a2, have previously been assigned to rat chromosome 13. Except for Ren, none of the loci have previously been mapped by linkage analysis. The markers for these loci were characterized in a total of 13 inbred rat strains (F344/N, LEW/N, LOU/MN, WBB1/N, WBB2/N, MR/N, MNR/N, ACI/N, SHR/N, WKY/N, BN/SsN, BUF/N, and LER/N) and were found to be highly polymorphic, with two to eight alleles detected for each marker. These markers expand the genetic map of the rat and should be valuable tools for future genetic studies. An examination of human and mouse comparative map information for all loci assigned to rat chromosome 13 shows significant synteny conservation with the q arm of human chromosome 1 and the distal portion of mouse chromosome 1.