Nitration enhances the allergenic potential of proteins
Nitration enhances the allergenic potential of proteins
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DOI：
10.1159/000095296
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Duschl, A.
中科院分区：
文献类型：
--
作者：
Gruijthuijsen, Y. K.;Grieshuber, I.;Duschl, A.
背景：最近的研究表明，包括Bet v 1a在内的蛋白质因暴露在污染的城市空气中而被硝化。我们在体内模型中研究了体外硝化变应原的免疫原性和致敏性。方法：比较未经处理和硝化处理的卵清蛋白或Bet v1a对DO11.10或致敏BALB/c小鼠脾细胞的增殖和细胞因子分泌的刺激作用，以及对致敏小鼠诱导特异性免疫球蛋白(1g)G1、IgG2a和IgE的能力。此外，对桦树花粉过敏个体的血清进行了硝化Bet v1a特异性IgE和Ig G的分析。结果：与未修饰的变应原相比，硝酸盐刺激脾细胞后，其增殖反应、IL-5和干扰素-γ的产生均有增加。硝化变应原致敏小鼠血清中特异性IgE、lgG1和lgG2a水平高于未修饰变应原致敏小鼠血清。此外，在小鼠中观察到了针对无关的硝化变应原的抗体的交叉反应。我们还发现，在桦树花粉过敏患者的血清中，针对硝酸盐的功能性、特异性IgE比针对未经治疗的Bet v1a的IgE更高。结论：我们的研究结果表明，硝化作用增强了过敏反应，这可能是污染的城市环境中变态反应性疾病患病率增加的原因之一。版权所有(C)2006 S.Karger AG，巴塞尔。
Background: Recent investigations have shown that proteins, including Bet v 1a, are nitrated by exposure to polluted urban air. We have investigated immunogenic and allergenic properties of in vitro nitrated allergens in in vivo models. Methods: Untreated and nitrated samples of ovalbumin or Bet v la were compared for their ability to stimulate proliferation and cytokine secretion in splenocytes from DO11.10 or from sensitized BALB/c mice, and for their ability to induce specific immunoglobulin (1g)G1, IgG2a and IgE in sensitized mice. Additionally, sera from birch pollen-allergic individuals were analysed for IgE and IgG specific for nitrated Bet v 1a. Results: Upon splenocyte stimulation with nitrated as compared with unmodified allergens, proliferation as well as interleukin 5 and interferon-gamma production were enhanced. Sera of mice sensitized with nitrated allergens showed elevated levels of specific IgE, lgG1 and lgG2a, compared with sera from mice sensitized with unmodified allergens. Moreover, cross-reactivity of antibodies against unrelated, nitrated allergens was observed in mice. We also found higher amounts of functional, specific IgE against nitrated than against untreated Bet v 1a in sera from birch pollen-allergic patients. Conclusions: Our findings suggest that nitration enhances allergic responses, which may contribute to an increased prevalence of allergic diseases in polluted urban environments. Copyright (c) 2006 S. Karger AG, Basel.