Effect of acid etching time and technique on interfacial characteristics of the adhesive-dentin bond using differential staining
Effect of acid etching time and technique on interfacial characteristics of the adhesive-dentin bond using differential staining
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DOI：
10.1111/j.1600-0722.2004.00127.x
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发表时间：
2004-06-01
影响因子：
1.9
通讯作者：
Spencer, P
中科院分区：
文献类型：
--
作者：
Wang, Y;Spencer, P
使用全蚀刻方法的牙本质粘结被认为是技术敏感的。本研究的目的是使用差异染色技术来检查酸蚀变化对牙本质脱矿和粘接剂-牙本质粘结的界面结构的影响。使用具有35%磷酸凝胶的Single Bond粘合剂。从60颗拔除的未萌出的人类第三磨牙中取出咬合三分之一的牙冠。通过在水下用600粒度的SiC研磨牙本质30秒来产生硬化层。根据酸蚀时间将预备好的牙齿随机分为4组（第1组，10 s;第2组，15 s;第3组，30 s;第4组，60 s）。在每组中，蚀刻凝胶：（i）应用并铺展到牙本质表面，并保持不受干扰;（ii）在蚀刻过程中应用并轻轻搅拌;（iii）在不使用注射器分配尖端的情况下应用，并保持与上述相同的时间。冲洗后，根据制造商的说明书用粘合剂处理蚀刻的牙本质。3-5用切片机切下粘合剂/牙本质（a/d）界面的μ m薄切片，并用Goldner三色染色。用光学显微镜对每个准备好的牙齿的染色薄切片进行成像。显微镜下可见牙本质脱矿的深度、程度及a/d相互扩散区。互扩散区的厚度作为蚀刻时间的函数而增加。然而，蚀刻剂凝胶应用方法对牙本质脱矿有显著影响。虽然搅拌酸性凝胶有助于渗透和蚀刻到牙本质中，但不应推荐使用，尤其是对于较长的蚀刻时间。这些结果表明，酸蚀技术对牙本质脱矿和界面结构的轮廓有很大的影响。
Dentin bonding using the total-etch method has been claimed to be technique-sensitive. The aim of this study is to examine the effect of acid-etch variations on the dentin demineralization and interfacial structure of the adhesive-dentin bond using a differential staining technique. Single Bond adhesive with 35% phosphoric acid gel was used. The occlusal one-third of the crown was removed from 60 extracted, unerupted human third molars. Smear layers were created by abrading the dentin with 600 grit SiC under water for 30 s. The prepared teeth were randomly assigned to four groups according to etching time (Group 1, 10 s; Group 2, 15 s; Group 3, 30 s; Group 4, 60 s). In each group, the etching gel was: (i) applied and spread to the dentin surface and left to stand undisturbed; (ii) applied and gently agitated during etching; (iii) applied without using dispensing tips for the syringe and left for the same period as above. After rinsing, the etched dentin was then treated with the adhesive per manufacturers' instructions. 3-5 mum thin sections of the adhesive/dentin (a/d) interface were cut with a microtome and stained with Goldner's trichrome. Stained, thin sections from each prepared tooth were imaged with light microscopy. The depth and extent of dentin demineralization, and the a/d interdiffusion zone were clearly visible by this differential staining microtechnique. The thickness of the interdiffusion zone increased as a function of etching time. However, the etchant gel application methods have a significant influence on dentin demineralization. Although agitating acid gel facilitates the penetration and etching into dentin, it should not be recommended, especially for longer etching time. These results indicated that the etching technique has a large effect on the profile of both dentin demineralization and interfacial structure.