Effects of Sleeve Gastrectomy on Nonalcoholic Fatty Liver Disease in an Obese Rat Model
Effects of Sleeve Gastrectomy on Nonalcoholic Fatty Liver Disease in an Obese Rat Model
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DOI：
10.1007/s11695-017-3052-4
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发表时间：
2018-06
期刊：
影响因子：
2.9
通讯作者：
Kiminori Watanabe;M. Ohta;Hiroomi Takayama;Kazuhiro Tada;Yuki Shitomi;T. Kawasaki;Yuichiro Kawano;Y. Endo;Y. Iwashita;M. Inomata
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文献类型：
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作者：
Kiminori Watanabe;M. Ohta;Hiroomi Takayama;Kazuhiro Tada;Yuki Shitomi;T. Kawasaki;Yuichiro Kawano;Y. Endo;Y. Iwashita;M. Inomata
背景/目的腹腔镜袖状胃切除术（SG）是一种越来越多使用的减肥手术，据报道对非酒精性脂肪性肝病（NAFLD）有效。最近，据报道，法尼醇X受体（FXR）（其是胆汁酸（BA）的核受体）的激活有助于NAFLD的消退。然而，尚不清楚SG是否对肝脏中FXR的表达有影响。我们的目的是研究FXR及其相关因子在肝脏SG后的表达，并阐明FXR表达的变化与NAFLD在肥胖大鼠model.Methods30雄性Zucker脂肪大鼠分为三组：假手术（SO）控制，配对喂养（PF）控制，和SG之间的关系。术后8周检测各组大鼠代谢指标、血浆总BA、肝酶、肝甘油三酯（TG）含量、FXR及其相关因子小异源二聚体伴侣（SHP）和过氧化物酶体增殖物激活受体α（PPARα）mRNA表达。SG组的肝酶和TG显著低于SO组。SG组血浆BA水平显著高于SO组和PF组。SG组肝脏FXR、SHP和PPARα mRNA表达显著高于SO group.ConclusionsThese结果提示SG对NAFLD的影响与Zucker脂肪大鼠模型肝脏FXR通路的表达有关。
Background/AimLaparoscopic sleeve gastrectomy (SG) is an increasingly used bariatric surgery, which is reported to be effective for nonalcoholic fatty liver disease (NAFLD). Recently, activation of farnesoid X receptor (FXR), which is a nuclear receptor of bile acid (BA), was reported to contribute to the resolution of NAFLD. However, it is unclear whether SG has an effect on expression of FXR in the liver. We aimed to investigate the expression of FXR and its related factors in the liver after SG and to clarify the relationship between changes in FXR expression and NAFLD in an obese rat model.MethodsThirty male Zucker fatty rats were divided into three groups: sham-operated (SO) control, pair-fed (PF) control, and SG. Eight weeks after the surgery, metabolic parameters, plasma levels of total BA and liver enzymes, liver triglyceride (TG) content, and mRNA expression of FXR and its related factors, such as small heterodimer partner (SHP) and peroxisome proliferator-activated receptor α (PPARα), were measured.ResultsMetabolic parameters in the SG group were significantly improved compared with the SO group. Liver enzymes and TG were significantly lower in the SG group than in the SO group. Plasma levels of BA were significantly higher in the SG group than in the SO and PF groups. mRNA expression of FXR, SHP, and PPARα in the liver was significantly higher in the SG group than in the SO group.ConclusionsThese results suggest that the effects of SG on NAFLD should be associated with the expression of the FXR pathway in the liver in a Zucker fatty rat model.