Solid phase synthesis of somatostatin-28.
Solid phase synthesis of somatostatin-28.
复制标题
生长抑素28的固相合成。
DOI：
10.1016/0006-291x(80)91564-8
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Ling,N;Esch,F;Davis,D;Mercado,M;Regno,M;Bohlen,P;Brazeau,P;Guillemin,R
用固相合成法合成了绵羊下丘脑生长抑素-28(Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH)。(1)用Beckman°C测序仪进行直接序列分析；(2)将分离的胰蛋白酶多肽片段的氨基酸分析与其理论组成相关联；(3)用高效液相色谱法将合成的甲硫氨酸-亚砜和甲硫氨酸-磺酸修饰的氨基末端多肽(残基1-11)与生长抑素-28的相应胰酶片段进行比较。
The synthesis of ovine hypothalamic somatostatin-28 (Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH) has been accomplished by solid phase methodology. The structure of the synthetic material was verified by:(1) direct sequence analysis with a Beckman 89° C sequencer,(2) correlation of the amino acid analyses of the isolated tryptic peptide fragments with their theoretical compositions, and (3) comparison, using high performance liquid chromatography, of the synthetic methionine-sulfoxide and methionine-sulfone modified NH 2-terminal peptides (residues 1–11) with the corresponding tryptic fragment from somatostatin-28.