I-menthol-induced [Ca2+]i increase and impulses in cultured sensory neurons
I-menthol-induced [Ca2+]i increase and impulses in cultured sensory neurons
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DOI：
10.1097/00001756-200007140-00018
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发表时间：
2000-07-14
期刊：
影响因子：
1.7
通讯作者：
Kobayashi, S
中科院分区：
文献类型：
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作者：
Okazawa, M;Terauchi, T;Kobayashi, S
我们研究了 L-薄荷醇对培养的背根神经节 (DRG) 细胞（而不是感觉纤维的游离神经末梢）的影响。使用 Fura-2 微荧光测定法，我们鉴定了一些 DRG 神经元，这些神经元表现出对 l-薄荷醇的反应，细胞内游离 Ca2+ 浓度 ([Ca2+](i)) 增加。它们仅占被高 K+ 溶液激活的神经元的 10%。 l-薄荷醇以剂量依赖性方式诱导[Ca2+](i)增加，EC50为37.9μM，Hill系数为0.97。相关化合物环己醇没有效果。当细胞外 Ca2+ 被去除时，l-薄荷醇不会诱导 [Ca2+](i) 增加。全细胞电流钳记录显示，l-薄荷醇诱导去极化（13.2 mV，受体电位），从而产生脉冲。我们得出结论，l-薄荷醇通过激活一小部分培养的感觉神经元中的薄荷醇受体来诱导冲动。 NeuroReport 11：2151-2155 (C) 2000 Lippincott Williams & Wilkins。
We investigated the effects of l-menthol on cultured dorsal root ganglion (DRG) cells, instead of free nerve endings of sensory fibers. Using Fura-2 microfluorimetry, we identified a few DRG neurons that showed an increase in intracellular free Ca2+ concentration ([Ca2+](i)) in response to l-menthol. They made up only 10% of the neurons activated by a high K+ solution. l-Menthol induced the [Ca2+](i) increase in a dose-dependent manner, with an EC50 of 37.9 mu M and a Hill coefficient of 0.97. A related compound, cyclohexanol, had no effect. When extracellular Ca2+ was removed, l-menthol did not induce the [Ca2+](i) increase. Whole-cell current-clamp recordings revealed that l-menthol induced depolarization (13.2 mV, receptor potential) leading to impulses. We conclude that l-menthol induced the impulses through activation of menthol receptors in a small subset of the cultured sensory neurons. NeuroReport 11:2151-2155 (C) 2000 Lippincott Williams & Wilkins.