Detection of Mycobacterium tuberculosis complex with Real Time PCR:: Comparison of different primer-probe sets based on the IS6110 element
Detection of Mycobacterium tuberculosis complex with Real Time PCR:: Comparison of different primer-probe sets based on the IS6110 element
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DOI：
10.1016/j.mimet.2005.12.003
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发表时间：
2006-07-01
影响因子：
2.2
通讯作者：
Noordhoek, Gerda T.
中科院分区：
文献类型：
--
作者：
Savelkoul, Paul H. M.;Catsburg, Arnold;Noordhoek, Gerda T.
比较了4套实时定量聚合酶链式反应(Real-Time PCR)对结核分枝杆菌复合体检测的敏感性和特异性。三组靶向IS6110序列上几乎相同的位置，SET 4靶向IS6110下游200bp的位置。用组1、组2和组3进行实时定量PCR，并以修饰靶的共扩增作为内部扩增对照。通过对相同的DNA样本进行测试，结果表明，在分析灵敏度和特异性方面，引物和探针序列的微小变化会导致分析结果的不同。(C)2005 Elsevier B.V.保留所有权利。
The sensitivity and specificity to detect Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the IS6110 sequence and set 4 targeted a location 200 bp downstream on IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity. (c) 2005 Elsevier B.V. All rights reserved.