High-performance liquid chromatography method for the determination of protein-glutathione mixed disulfide.
High-performance liquid chromatography method for the determination of protein-glutathione mixed disulfide.
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高效液相色谱法测定蛋白质-谷胱甘肽混合二硫化物。
DOI：
10.1006/abio.1994.1352
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发表时间：
1994
影响因子：
2.9
通讯作者：
Srivastava,SK
中科院分区：
文献类型：
--
作者：
Kumari,K;Khanna,P;Ansari,NH;Srivastava,SK
我们建立了一种有效和灵敏的方法定量测定皮摩尔量的蛋白质-谷胱甘肽和蛋白质-半胱氨酸混合二硫化物的透镜。过甲酸氧化从蛋白质混合的二硫化物中释放谷胱甘肽和半胱氨酸，分别作为谷胱甘肽磺酸和半胱氨酸。磺酸用异硫氰酸苯酯衍生化，苯硫代氨基甲酰基衍生物用反相ODS柱通过HPLC分离，该柱用含有0.1%三乙胺和6%乙腈的0.14 M乙酸钠平衡。
We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.