Carbon isotopic fractionation by Archaeans and other thermophilic prokaryotes
Carbon isotopic fractionation by Archaeans and other thermophilic prokaryotes
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DOI：
10.1016/s0146-6380(02)00237-1
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发表时间：
2003-01-01
影响因子：
3
通讯作者：
Stetter, KO
中科院分区：
文献类型：
--
作者：
House, CH;Schopf, JW;Stetter, KO
这项对21种系统发育不同的微生物物种的碳同位素分馏的研究提供了一个将碳同位素分馏与生化途径联系起来的机会。这些碳同位素分馏实验包括两个Aquificales成员和两个Thermoproteales成员使用还原性TCA循环，三个Sulfolobales成员使用3-羟基丙酸循环，以及三个archaelobales成员和七个甲烷生菌使用乙酰辅酶a途径。在这些实验中，使用还原型三羧酸循环（6值在2.0至5.5‰之间）和3-羟丙酸循环（epsilon值在0.2至3.6‰之间）的微生物的碳同位素分馏明显少于使用乙酰辅酶a途径的产甲烷菌。然而，本文报道的利用乙酰辅酶a途径的微生物的结果变化范围非常大，古球菌的epsilon值为2.7至8.0‰，甲烷菌的epsilon值为4.8至26.7‰。在甲烷球菌物种中观察到的碳同位素分馏的大小与不同培养物所达到的特定生长状态有关，随着生长的进行，同位素分馏的增加。(C) 2002 Elsevier Science Ltd.版权所有。
This study of carbon isotopic fractionation in a wide array of 21 phylogenetically diverse microbial species provides an opportunity to correlate carbon isotopic fractionations with a biochemical pathway. These carbon isotopic fractionation experiments included two members of the Aquificales and two members of the Thermoproteales using the reductive TCA cycle, three members of the Sulfolobales using the 3-hydroxypropionate cycle, as well as three Archaeoglobales and seven methanogens using the acetyl-CoA pathway. In these experiments, microorganisms using the reductive tricarboxylic acid cycle (with 6 values between 2.0 and 5.5parts per thousand) and the 3-hydroxypropionate cycle (with epsilon values between 0.2 and 3.6parts per thousand) demonstrated significantly less carbon isotopic fractionation than methanogens using the acetyl-CoA pathway. The results reported here for the acetyl-CoA pathway-utilizing microbes, however, vary over a remarkably wide range with epsilon values of 2.7 to 8.0parts per thousand for the Archaeoglobales and epsilon values of 4.8 to 26.7parts per thousand for the methanogens. The magnitude of carbon isotopic fractionation observed in species of Methanococcus were related to the particular growth status that had been attained by the various cultures, with increasing isotopic fractionation as growth proceeded. (C) 2002 Elsevier Science Ltd. All rights reserved.