Mathematical Modeling of Protectant Transport in Tissues.
Mathematical Modeling of Protectant Transport in Tissues.
复制标题
DOI：
10.1007/978-1-0716-0783-1_5
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Higgins AZ
中科院分区：
文献类型：
--
作者：
Warner RM;Higgins AZ
保护剂化学品的传质是低温保存和冷冻干燥方案的基本方面。因此，传质建模对于保存方法的设计是有用的。细胞膜转运模型已成功地用于指导分离细胞保存方法的设计。然而，对于组织，存在由与细胞嵌入组织基质中相关的现象引起的若干传质建模挑战。细胞和组织基质都形成了水和保护性化学物质自由扩散的屏障。值得注意的是，细胞外空间对建模变得重要。包埋在组织中的细胞的反应取决于在空间和时间上变化的细胞外空间的状态。细胞外空间中的运输也可导致组织大小的变化。在这一章中，我们描述了各种传质模型，可用于描述运输过程中发生的现象与保护性分子的冷冻保存应用的组织加载。限制这些模型的适用性的假设和简化进行了讨论。
Mass transfer of protectant chemicals is a fundamental aspect of cryopreservation and freeze drying protocols. As such, mass transfer modeling is useful for design of preservation methods. Cell membrane transport modeling has been successfully used to guide design of preservation methods for isolated cells. For tissues, though, there are several mass transfer modeling challenges that arise from phenomena associated with cells being embedded in a tissue matrix. Both cells and the tissue matrix form a barrier to the free diffusion of water and protective chemicals. Notably, the extracellular space becomes important to model. The response of cells embedded in the tissue is dependent on the state of the extracellular space which varies both spatially and temporally. Transport in the extracellular space can also lead to changes in tissue size. In this chapter, we describe various mass transfer models that can be used to describe transport phenomena occurring during loading of tissues with protective molecules for cryopreservation applications. Assumptions and simplifications that limit the applicability of each of these models are discussed.