Development of the cerebellum in turbot (Psetta maxima): Analysis of cell proliferation and distribution of calcium binding proteins
Development of the cerebellum in turbot (Psetta maxima): Analysis of cell proliferation and distribution of calcium binding proteins
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DOI：
10.1016/j.jchemneu.2017.07.001
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发表时间：
2017-11-01
影响因子：
2.8
通讯作者：
Alvarez-Otero, Rosa
中科院分区：
文献类型：
--
作者：
De Miguel, Encarnacion;Alvarez-Otero, Rosa
应用常规组织学技术和增殖细胞核抗原及钙结合蛋白的免疫组织化学方法，对大菱鲆（Psettamimax）小脑的形态发生、细胞增殖和神经元分化进行了研究。与其他脊椎动物一样，小脑原基在胚胎期以神经组织增殖板的形式出现。小脑的原基持续没有形态学的变化，直到幼虫生命的结束时，地幔区分化。主要的个体发育变化，驱动小脑亚部的形成开始在后期前变质幼虫时，小脑板的增长和合并的中间。这种转化是通过增殖区的重组以及细胞分化的开始来完成的。小脑成为完全分化变态过程中检测到小白蛋白和钙蛋白在浦肯野细胞和eurydendroidoid细胞。持续的增殖保持在小脑的所有分区，这支持这部分大脑的发生在变质和少年periods.The位置和组织学组织的增殖活动在大菱鲆成熟小脑的强劲增长，并根据其他硬骨鱼可用的信息，分析其功能意义。(C)2017爱思唯尔B.V.保留所有权利。
The morphogenesis, cell proliferation and neuronal differentiation of the turbot (Psetta maxima) cerebellum has been studied using conventional histological techniques and immunohistochemical methods for proliferating cell nuclear antigen and calcium binding proteins. As in other vertebrates, the cerebellar anlage emerges as proliferative plates of neural tissue during the embryonic period. The anlage of the cerebellum persists without morphological changes until the end of the larval life when the mantle zone is differentiated. The major ontogenetic changesthat drive the formation of the cerebellar subdivisions begin in late premetamorphic larvae when cerebellar plates growth and merge medially. This transformation is accomplished by the reorganization of proliferative zones as well as by the onset of cell differentiation. The cerebellum becomes fully differentiated during metamorphosis when parvalbumin and calretinin were detected in Purkinje and eurydendroid cells. Sustained proliferation is maintained in all subdivisions of the cerebellum and this support the robust growth of this part of the brain that takes place during the metamorphic and juvenile periods.The location and histological organization of the proliferative activity in the turbot mature cerebellum are described and their functional significance was analyzed in light of the information available for other teleosts. (C) 2017 Elsevier B.V. All rights reserved.