Genetic diversity among Campylobacter jejuni isolates from pets in Ireland
Genetic diversity among Campylobacter jejuni isolates from pets in Ireland
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DOI：
10.1136/vr.c357
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发表时间：
2010-01-23
影响因子：
2.2
通讯作者：
Whyte, P.
中科院分区：
文献类型：
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作者：
Acke, E.;McGill, K.;Whyte, P.
通过fla分型（使用Ddel和Hinfl限制性内切酶）和脉冲场凝胶电泳（PFGE）技术对从收容所和私人家庭饲养的宠物中分离的空肠弯曲杆菌进行分型。78株C型空肠分离株的复合fla聚类分析具有较好的判别性（39.7%）。对52株C型空肠分离株的PFGE分析结果显示，53.8%为单分离株，是最具鉴别性的方法。建立了爱尔兰宠物C型空肠菌亚型数据库。在空肠C亚型中检测到的遗传异质性表明，宠物可以从多种潜在来源获得生物体。此外，在同一只宠物中，不同培养方法获得的空肠C亚型存在异质性。在同一环境中密切接触的狗的分离株之间存在联系，证实这是一种潜在的感染途径，并且发现了包含猫和狗C空肠分离株的聚集性，表明可能存在种间传播。
Campylobacter jejuni isolates obtained from pets housed in shelters and in private households were subtyped by fla typing (using Ddel and Hinfl restriction enzymes) and pulsed-field gel electrophoresis (PFGE) techniques. Composite fla cluster analysis on 78 C jejuni isolates was more discriminative than either single fla typing technique with 39.7 per cent single isolate patterns. PFGE on 52 C jejuni isolates revealed 53.8 per cent single isolate patterns and was the most discriminative method applied. A database of C jejuni subtyping profiles from pets in Ireland was assembled. The presence of genetic heterogeneity detected in the C jejuni subtypes suggests that pets can acquire the organisms from multiple potential sources. in addition, heterogeneity was detected in the C jejuni subtypes obtained by different culture methods within the same pet. There was a link between isolates from dogs in close contact in the same environment, confirming that this is a potential route of infection, and clusters were detected containing both cat and dog C jejuni isolates, suggesting possible interspecies transmission.