Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All‐Female Japanese Flounder (Paralichthys Olivaceus)
Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All‐Female Japanese Flounder (Paralichthys Olivaceus)
复制标题
DOI：
10.1002/ar.23698
复制
发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Yang Yang-Yang;Qinghua Liu;Yongshuang Xiao;Xueying Wang;Hao An;Z. Song;F. You;Yanfeng Wang;D. Ma;Jun Li
中科院分区：
文献类型：
--
作者：
Yang Yang-Yang;Qinghua Liu;Yongshuang Xiao;Xueying Wang;Hao An;Z. Song;F. You;Yanfeng Wang;D. Ma;Jun Li
在这项研究中，日本比目鱼的所有遗传雌性 (XX) 幼仔都是通过雌性与性别逆转的雄性交配而人工产生的。详细描述了原始生殖细胞（PGC）的增殖和迁移、卵巢的形成和卵子发生。孵化后，大约20个单独的PGC从躯干区域的侧面迁移到背侧。孵化后 15 天 (dph)，部分 PGC 被单层体细胞覆盖并形成生殖嵴。 22 dph 时，细长的性腺原基出现在腹肾下方，其中 PGC 完全被体细胞包围。在迁移过程中，PGC 被认为是不活跃有丝分裂的。从 63 dph 到 73 dph，体细胞重排导致狭窄裂缝的形成，该裂缝变得更深并形成卵巢腔。然而，在 52 dph 时，生殖细胞发生剧烈的有丝分裂增殖，并在卵巢管腔出现之前形成生殖系巢。密集的生殖细胞增殖的开始和细胞巢的出现可以被接受作为初始卵巢分化的标准。然后生殖细胞和体细胞上皮细胞逐渐被基底膜分隔，形成生发上皮。这一时期，原位杂交结果显示，早期叉头盒L2（pofoxl2）在初级生长的体细胞和卵母细胞中表达，这表明卵泡前细胞已形成。然后卵原细胞或卵母细胞、卵泡细胞、基底膜和卵泡膜细胞组成卵泡复合体。最后，卵母细胞经历减数分裂并发育成成熟的卵子。 Anat Rec，301:727–741，2018。© 2017 Wiley periodicals, Inc.
In this study, all genetic female (XX) broods of Japanese flounder were produced artificially by mating the females with sex‐reversed males. The proliferation and migration of primordial germ cells (PGCs), formation of ovary and oogenesis were described in detail. After hatching, around 20 individual PGCs migrated from the lateral to the dorsal of trunk region. At 15 days posthatching (dph), a part of PGCs were covered by a single layer somatic cells and formed the genital ridge. By 22 dph, the elongated gonadal primordia appeared under the ventral kidney, where the PGCs were totally enclosed by somatic cells. During the process of migration, PGCs were presumed to be mitotically inactive. From 63 to 73 dph, somatic cells rearrangement resulted in the formation of a narrow crevice, which became deeper and formed ovarian lumen. However, at 52 dph, dramatic mitotic proliferation of germ cell occurred and germline nest formed before the appearance of ovarian lumen. The onset of intensive germ cell proliferation and appearance of cell nests could be accepted as a criterion of initial ovarian differentiation. Then germ cells and somatic epithelial cells were gradually delimited by basement membrane and formed the germinal epithelium. In this period, results from in situ hybridization revealed that the early forkhead box L2 (pofoxl2) was expressed in somatic cells and oocytes in primary growth, which indicated the prefollicle cells formed. Then oogonia or oocytes, follicle cells, basement membrane, and theca cells composed a follicle complex. Finally, oocytes underwent meiosis and developed into to mature eggs. Anat Rec, 301:727–741, 2018. © 2017 Wiley Periodicals, Inc.