Influence of the redox state of pyridine nucleotides on mitochondrial sulfhydryl groups and permeability transition
Influence of the redox state of pyridine nucleotides on mitochondrial sulfhydryl groups and permeability transition
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DOI：
10.1006/abbi.1997.9986
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发表时间：
1997-06-01
影响因子：
3.9
通讯作者：
Rigobello, MP
中科院分区：
文献类型：
--
作者：
Bindoli, A;Callegaro, MT;Rigobello, MP
这项工作解决了吡啶核苷酸的氧化还原状态和线粒体膜的巯基之间的相关性。出现了几个主要意见：（1）导致吡啶核苷酸氧化的条件，例如与叔丁基过氧化氢或乙酰乙酸盐孵育，决定了总线粒体巯基的减少。谷氨酸不遵循相同的模式，因为它在叔丁基过氧化氢的存在下降低，但在乙酰乙酸的存在下不降低。此外，只有在叔丁基过氧化氢的存在下，巯基的减少伴随着膜蛋白聚合，通过聚丙烯酰胺凝胶电泳观察到。(2)在所有测试条件下，巯基的氧化进一步受到钙离子和磷酸根离子的刺激。(3)防止线粒体肿胀的呼吸底物也部分防止巯基的减少。(C)北京：科学出版社.
This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determine a decrease of total mitochondrial sulfhydryl groups. Glutathione does not follow the same pattern since it decreases in the presence of tert-butyl hydroperoxide but not in the presence of acetoacetate. In addition, only in the presence of tert-butyl hydroperoxide is the decrease of sulfhydryl groups concomitant with a membrane protein polymerization, observed by polyacrylamide gel electrophoresis. (2) Under all conditions tested, the oxidation of sulkydryl groups is further stimulated by the presence of calcium and phosphate ions. (3) Respiratory substrates, which prevent the swelling of mitochondria, also partially prevent the decrease of sulfhydryl groups. (C) 1997 Academic Press.