Connexins 26 and 30 are co-assembled to form gap junctions in the cochlea of mice
Connexins 26 and 30 are co-assembled to form gap junctions in the cochlea of mice
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DOI：
10.1016/s0006-291x(03)01166-5
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发表时间：
2003-07-25
影响因子：
3.1
通讯作者：
Lin, X
中科院分区：
文献类型：
--
作者：
Ahmad, S;Chen, SP;Lin, X
连接蛋白（Cxs）在耳蜗功能中的重要性已被发现，连接蛋白基因的突变导致了很大比例的感音神经性耳聋病例。然而，连接蛋白在耳蜗中的功能作用仍不清楚。在这项研究中，我们比较了16种不同亚型的小鼠连接蛋白在耳蜗中的相对表达水平。cDNA宏阵列杂交鉴定了四种最显著表达的连接蛋白（按降序列出）：Cxs 26、29、30和43。其中两个连接蛋白（Cx26和COO），都属于β组，研究了它们在耳蜗中的分子组装。共免疫染色显示Cxs 26和30在相同的间隙连接斑块中表达，并且通过从耳蜗组织提取的蛋白质的共免疫沉淀证实了它们的共组装。预期连接蛋白的异源分子组装产生具有生物物理特性的间隙连接，其适于维持耳蜗中的离子稳态。(C)2003年爱思唯尔公司All rights reserved.
The importance of connexins (Cxs) in the cochlear functions has been indicated by the finding that mutations in connexin genes cause a large proportion of sensorineural deafness cases. However, functional roles of connexins in the cochlea are still unclear. In this study, we compared the relative expression levels of 16 different subtypes of mouse connexins in the cochlea. cDNA macroarray hybridizations identified four most prominently expressed connexins (listed in descending order): Cxs 26, 29, 30, and 43. Two of these connexins (Cx26 and COO), both belonging to the beta-group, were investigated for their molecular assemblies in the cochlea. Co-immunostaining showed expressions of Cxs 26 and 30 in the same gap junction plaques and their co-assembly was confirmed by co-immunoprecipitation of proteins extracted from the cochlear tissues. The heterologous molecular assembly of connexins is expected to produce gap junctions with biophysical characteristics appropriate for maintaining ionic homeostasis in the cochlea. (C) 2003 Elsevier Inc. All rights reserved.