Circular dichroism for the determination of amphotericin B binding to liposomes.
Circular dichroism for the determination of amphotericin B binding to liposomes.
复制标题
用于测定两性霉素 B 与脂质体结合的圆二色性。
DOI：
10.1016/0003-2697(88)90432-0
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发表时间：
1988
影响因子：
2.9
通讯作者：
Bolard,J
中科院分区：
文献类型：
--
作者：
Jullien,S;Vertut-Croquin,A;Brajtburg,J;Bolard,J
抗真菌抗生素阿朴霉素B（AmB）的圆二色性（CD）可用于表征药物的脂质体制剂中与脂质结合的药物水平。在水中游离Amstericin B以340 nm为中心的非常强的二色性双峰或在435 nm以上观察到的二色性可用于测定这些载体系统中含有高抗生素/脂质比（范围为10 - 2至10 - 1）的制剂中结合AmB和游离AmB的百分比。实例中给出的AmB的存在下制备的四个饱和脂肪酰基链磷脂酰胆碱的不同链长，有或没有胆固醇的小单层囊泡。AmB从囊泡转移到两种血液成分，血清白蛋白和脂蛋白也可以在特定条件下通过CD监测。
Circular dichroism (CD) of the antifungal antibiotic amphotericin B (AmB) can be used to characterize the liposomal preparations of the drug with regard to the levels of drug bound to the lipids. The very intense dichroic doublet centered around 340 nm of free amphotericin B in water or the dichroism observed above 435 nm can be used to determine the percentages of bound AmB and free AmB in preparations containing high antibiotic/lipid ratios (ranging from 10−2to 10−1) used in these carrier systems. Examples are given for AmB in the presence of small unilamellar vesicles prepared from four saturated fatty acyl chain phosphatidylcholines of different chain lengths, with or without cholesterol. The transfer of AmB from vesicles to two blood components, serum albumin, and lipoproteins can also be monitored by CD under particular conditions.