Localization, transport, and uptake of D-aspartate in the rat adrenal and pituitary glands.
Localization, transport, and uptake of D-aspartate in the rat adrenal and pituitary glands.
复制标题
D-天冬氨酸在大鼠肾上腺和垂体中的定位、运输和摄取。
DOI：
10.1006/abbi.2000.2163
复制
发表时间：
2001
影响因子：
3.9
通讯作者：
H. Homma
中科院分区：
文献类型：
--
作者：
J. A. Lee;Z. Long;N. Nimura;T. Iwatsubo;K. Imai;H. Homma
大鼠肾上腺和脑垂体中存在大量d -天冬氨酸（D-Asp）。D-Asp被认为是在哺乳动物体内合成的，并在腹腔或静脉给药后在各种组织中积累。本报告探讨了肾上腺和脑垂体中D-Asp的来源。我们给雄性大鼠腹腔注射D-Asp，并使用我们实验室先前开发的抗D-Asp抗血清将这种外源性D-Asp免疫定位于肾上腺和垂体组织。大鼠肾上腺中的D-Asp水平在3周龄时短暂升高，此后迅速下降。我们发现，在肾上腺中，腹腔内给药的外源性D-Asp与内源性D-Asp存在于肾上腺皮质的同一区域。通过Northern和Western blot分析和谷氨酸转运蛋白的免疫组化，我们还发现，与D-Asp有亲和力的谷氨酸转运蛋白（Glu transporter， GLAST）的表达在3周龄时短暂增加，并且Glu转运蛋白在组织内的定位模式与内源性D-Asp的定位模式几乎一致。这些观察结果表明，3周龄雄性大鼠肾上腺皮质中的D-Asp主要通过血管系统摄取获得。我们之前已经证明D-Asp特异性定位于成年大鼠脑垂体前叶含有催乳素（PRL）的细胞中。在这里，我们报道了在垂体中，外源性D-Asp在内皮细胞中积累，而不是在prl细胞中积累。Northern和Western blot分析以及Glu转运蛋白的免疫组化分析显示，发育过程中Glu转运蛋白（GLAST）的表达变化与组织中D-Asp水平无关，且Glu转运蛋白在prl细胞中不表达。这些观察结果表明，与肾上腺相反，成年雄性大鼠脑垂体中的大部分D-Asp来源于腺体本身。
Large amounts of D-aspartate (D-Asp) are present in the rat adrenal and pituitary glands. D-Asp is thought to be synthesized in the mammalian body and also accumulates in various tissues following intraperitoneal or intravenous administration. This report examines the origins of D-Asp in the adrenal and pituitary glands. We administered D-Asp to male rats intraperitoneally and immunolocalized this exogenous D-Asp in adrenal and pituitary tissue, using an anti-D-Asp antiserum which was previously developed in our laboratory. D-Asp levels in the rat adrenal gland have been shown to undergo a transient increase at 3 weeks of age and to decrease rapidly thereafter. We found that in the adrenal gland, exogenous D-Asp administered intraperitoneally was incorporated into the same region of the adrenal cortex in which endogenous D-Asp was present. By Northern and Western blot analysis and immunohistochemistry of glutamate (Glu) transporter, we also found that expression of the Glu transporter (GLAST), which has an affinity for D-Asp, transiently increased at 3 weeks of age and that localization patterns of the Glu transporter within the tissue were almost coincident with those of endogenous D-Asp. These observations suggest that D-Asp in the adrenal cortex of 3-week-old male rats is primarily acquired by uptake from the vascular system. We have previously shown that D-Asp is specifically localized in prolactin (PRL)-containing cells in the anterior lobe of the adult rat pituitary gland. Here we report that in the pituitary gland, exogenous D-Asp accumulated in endothelial cells, but not in PRL-containing cells. Northern and Western blot analysis and immunohistochemistry of Glu transporter revealed that developmental changes in the Glu transporter (GLAST) expression did not correlate with tissue levels of D-Asp and that the Glu transporter was not expressed in PRL-containing cells. These observations suggest that, in contrast to the adrenal gland, most of the D-Asp in the pituitary gland of adult male rats originates inside the gland itself.
DOI：
10.1073/pnas.96.2.721
发表时间：
1999-01-19
影响因子：
11.1
作者：
Wolosker, H;Sheth, KN;Snyder, SH
通讯作者：
Snyder, SH
DOI：
10.1073/pnas.96.23.13409
发表时间：
1999-11-09
影响因子：
11.1
作者：
Wolosker, H;Blackshaw, S;Snyder, SH
通讯作者：
Snyder, SH
DOI：
10.1073/pnas.94.5.2013
发表时间：
1997-03-04
影响因子：
11.1
作者：
Schell, MJ;Cooper, OB;Snyder, SH
通讯作者：
Snyder, SH