RAPID DETECTION OF VIRUS GENOME FROM IMPORTED DENGUE FEVER AND DENGUE HEMORRHAGIC-FEVER PATIENTS BY DIRECT POLYMERASE CHAIN-REACTION
RAPID DETECTION OF VIRUS GENOME FROM IMPORTED DENGUE FEVER AND DENGUE HEMORRHAGIC-FEVER PATIENTS BY DIRECT POLYMERASE CHAIN-REACTION
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DOI：
10.1002/jmv.1890440111
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发表时间：
1994-09-01
影响因子：
12.7
通讯作者：
IGARASHI, A
中科院分区：
文献类型：
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作者：
MORITA, K;MAEMOTO, T;IGARASHI, A
对输入性登革热和登革出血热患者血清标本直接进行逆转录聚合酶链反应（RT-PCR），不经任何RNA纯化。扩增的病毒基因组在3小时内被检测到。PCR结果与蚊细胞分离病毒结果一致，表明直接RT-PCR方法能快速诊断登革热感染。(C)1994 Wiley-Liss，Inc.
Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection. (C) 1994 Wiley-Liss, Inc.