Lipopolysaccharide induces autotaxin expression in human monocytic THP-1 cells
Lipopolysaccharide induces autotaxin expression in human monocytic THP-1 cells
复制标题
脂多糖诱导人单核细胞 THP-1 细胞中自分泌运动因子的表达
DOI：
10.1016/j.bbrc.2008.11.047
复制
发表时间：
2009-01-09
影响因子：
3.1
通讯作者：
Zhang, Junjie
中科院分区：
文献类型：
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作者：
Li, Song;Zhang, Junjie
自分泌运动因子（Autotaxin，ATX）是一种具有溶血磷脂酶D（lysoPLD）活性的分泌型酶，能将溶血磷脂酰胆碱（LPC）转化为溶血磷脂酸（LPA），后者是一种具有生物活性的磷脂，参与细胞增殖、分化和迁移等多种生物活性。在本研究中，我们发现，细菌脂多糖（LPS），一个众所周知的炎症反应的引发剂，诱导ATX单核细胞THP-1细胞的表达。ATX诱导需要PKR、JNK和p38 MAPK的激活。LPS诱导的ATX III THP-1细胞被表征为β同种型。在LPC存在下，ATX可促进THP-1和Jurkat细胞的迁移，而这一作用可被百日咳毒素（PTX）抑制，百日咳毒素是一种Gi介导的LPA受体信号传导的抑制剂。综上所述，LPS通过PKR、JNK和p38 MAPK介导的机制诱导THP-1细胞中ATX的表达，ATX诱导可能通过调节微环境中的LPA水平来增强促炎反应中的免疫细胞迁移。(C)2008年爱思唯尔公司All rights reserved.
Autotaxin (ATX) is a secreted enzyme with lysophospholipase D (lysoPLD) activity, which converts lysophosphatidylcholine(LPC) into lysophosphatidic acid (LPA), a bioactive phospholipid involved ill numerous biological activities, including cell proliferation, differentiation, and migration. In the present study, we found that bacterial lipopolysaccharide(LPS), a well-known initiator of the inflammatory response, induced ATX expression in monocytic THP-1 cells. The activation of PKR, JNK, and p38 MAPK was required for the ATX induction. The LPS-induced ATX ill THP-1 cells was characterized as the beta isoform. In the presence of LPC, ATX could promote the migrations of THP-1 and Jurkat cells, which was inhibited by pertussis toxin (PTX), an inhibitor of Gi-mediated LPA receptor signaling, In summary, LPS induces ATX expression ill THP-1 cells via a PKR, JNK and p38 MAPK-mediated mechanism, and the ATX induction is likely to enhance immune cell migration in proinflammatory response by regulating LPA levels in the microenvironment. (C) 2008 Elsevier Inc. All rights reserved.