Quantitating protein synthesis, degradation, and endogenous antigen processing
Quantitating protein synthesis, degradation, and endogenous antigen processing
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DOI：
10.1016/s1074-7613(03)00051-7
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发表时间：
2003-03-01
期刊：
影响因子：
32.4
通讯作者：
Yewdell, JW
中科院分区：
文献类型：
--
作者：
Princiotta, MF;Finzi, D;Yewdell, JW
使用L929细胞，我们定量蛋白质合成和降解的宏观经济学和从病毒翻译产物生产MHC I类相关肽的微观经济学。为了维持2.6 × 10（9）个蛋白质的含量，每个细胞的6 × 10（6）个核糖体每分钟产生4 × 10（6）个蛋白质。每个细胞的8 × 105个蛋白酶体每分钟降解2.5个底物，每降解500-3000个病毒翻译产物产生一个MHC I类肽复合物。复合物形成的效率在树突状细胞和巨噬细胞中是相似的，它们在体内活化T细胞中起关键作用。蛋白酶体从两种不同的底物池中以不同的效率产生抗原肽：快速降解的新合成的蛋白质，其清楚地代表有缺陷的核糖体产物（DRiPs）和降解不太快的池，其中DRiPs也可能占主导地位。
Using L929 cells, we quantitated the macroeconomics of protein synthesis and degradation and the micro-economics of producing MHC class I associated peptides from viral translation products. To maintain a content of 2.6 x 10(9) proteins, each cell's 6 x 10(6) ribosomes produce 4 x 10(6) proteins min(-1). Each of the cell's 8 x 10(5) proteasomes degrades 2.5 substrates min(-1), creating one MHC class I-peptide complex for each 500-3000 viral translation products degraded. The efficiency of complex formation is similar in dendritic cells and macrophages, which play a critical role in activating T cells in vivo. Proteasomes create antigenic peptides at different efficiencies from two distinct substrate pools: rapidly degraded newly synthesized proteins that clearly represent defective ribosomal products (DRiPs) and a less rapidly degraded pool in which DRiPs may also predominate.