Preparation of tissues for DNA flow cytometric analysis.
Preparation of tissues for DNA flow cytometric analysis.
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用于 DNA 流式细胞术分析的组织的制备。
DOI：
10.1002/cyto.990010309
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Temple,WJ
中科院分区：
文献类型：
--
作者：
Thornthwaite,JT;Sugarbaker,EV;Temple,WJ
描述了一种通过流式细胞术利用一步组合核分离-DNA荧光染料染色程序测量组织细胞中DNA的方法。各种细胞和组织，体内和体外，被用来说明这种技术的通用性。这些包括小鼠骨髓、肝睾丸、肉瘤脑肿瘤、大鼠胰岛、人外周血、结肠粘膜、结肠癌、肉瘤和脑肿瘤组织。一种特殊的核分离介质，其中含有DNA荧光染料，4′，6-diamidino-2 phenylindole-2 HCl或碘化丙啶，成功地用于分离DNA荧光染料染色的细胞核的单一悬浮液，在快速（5-10分钟），从各种组织和细胞的一致的方式。相同组织的多次取样或整个组织与其单细胞分离株之间的比较表明获得了代表性样品。
A method for measuring DNA in tissue cells by flow cytometry utilizing a one step combination nuclear isolation‐DNA fluorochrome staining procedure is described. A variety of cells and tissues, bothin vivoandin vitro, was used to illustrate the universal nature of this technique. These included murine bone marrow, liver testicle, sarcoma brain tumor, rat pancreatic islets, human peripheral blood, colon mucosa, colon cancer, sarcoma and brain tumor tissues. A special nuclear isolation medium, which contained either of the DNA fluorochromes, 4′,6‐diamidino‐2 phenylindole‐2 HCl or propidium iodide, was utilized successfully to isolate single suspensions of DNA fluorochrome stained nuclei in a rapid (5–10 min), consistent manner from a variety of tissues and cells. Multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates showed that a representative sample was being obtained.