A novel non-enzymatic electrochemical biosensor based on the nanohybrid of bimetallic PdCu nanoparticles/carbon black for highly sensitive detection of H2O2 released from living cells
A novel non-enzymatic electrochemical biosensor based on the nanohybrid of bimetallic PdCu nanoparticles/carbon black for highly sensitive detection of H2O2 released from living cells
复制标题
一种基于双金属 PdCu 纳米颗粒/炭黑纳米杂化物的新型非酶电化学生物传感器，用于高灵敏度检测活细胞释放的 H2O2
DOI：
10.1016/j.snb.2019.03.129
复制
发表时间：
2019-07-01
影响因子：
8.4
通讯作者：
Zhao, Peng
中科院分区：
文献类型：
--
作者：
Liu, Yanyan;Li, Hongmei;Zhao, Peng
过氧化氢 (H2O2) 是生物体中氧的主要代谢物，称为活性氧 (ROS)。监测活细胞释放的H2O2具有重要意义，其浓度水平与多种疾病相关，这对检测技术的灵敏度提出了更严格的要求。在这项工作中，我们发现炭黑（CB）可以保留双金属PdCu合金的纳米结构，同时改善纳米颗粒的分散性，提供高电导率和大的比表面积，从而使PdCu/CB纳米杂化物表现出增强的H2O2电分析性能。在最佳条件下，PdCu/CB纳米杂化修饰电极表现出54 nM的低检测限和0.4 μM至5.0 mM的宽检测范围。最后，实现了PdCu/CB/GCE在实时监测Raw 264.7细胞释放的H2O2中的应用，表明PdCu/CB纳米杂化物是监测生理过程的有前途的纳米材料。
Hydrogen peroxide (H2O2) is a major metabolite of oxygen known as reactive oxygen species (ROS) in living organisms. Monitoring H2O2 released from living cells is of great significance as its concentration level is related to several diseases, which imposes stricter requirements on the sensitivity of detection technology. In this work, we have found that carbon black (CB) could preserve bimetallic PdCu alloy's nanostructure, meanwhile improve the dispersion of nanoparticles, provided high conductivity and large specific surface area, thus the PdCu/CB nanohybrid exhibited enhanced electroanalytical performance of H2O2. Under the optimal conditions, the PdCu/CB nanohybrid modified electrode showed a low detection limit of 54 nM with a wide detection range from 0.4 mu M to 5.0 mM. Finally, the application of PdCu/CB/GCE in real-time monitoring H2O2 released from Raw 264.7 cells was achieved, suggesting the PdCu/CB nanohybrid was the promising nanomaterial in monitoring physiological processes.