Species variation in the spontaneous calcification of bone marrow-derived mesenchymal stem cells
Species variation in the spontaneous calcification of bone marrow-derived mesenchymal stem cells
复制标题
骨髓间充质干细胞自发钙化的物种变异
DOI：
10.1016/j.jcyt.2012.11.011
复制
发表时间：
2013-03-01
期刊：
影响因子：
4.5
通讯作者：
Deng, Li
中科院分区：
文献类型：
--
作者：
Huang, Yi-Zhou;Cai, Jia-Qin;Deng, Li
骨髓间充质干细胞(BM-MSCs)具有良好的组织再生前景。随着越来越多的临床试验，骨髓间充质干细胞的安全性引起了人们的极大兴趣。此前，我们确定大鼠BM-MSCs在连续培养后有自发的钙化，没有成骨诱导。然而，目前还不清楚来自其他物种的BM-MSCs是否具有这一特征。本研究对大鼠、山羊和人骨髓间充质干细胞的体外自发钙化进行了研究。BM-MSCs在完全培养液中培养，通过形态观察和茜素红染色确定细胞的钙化程度。结果表明，大鼠BM-MSCs具有典型的自发性钙化，而在同一体系中，山羊和人BM-MSCs有明显的增殖，但不自发钙化。本研究中描述的BM-MSCs自发性钙化的显著种类差异为评估基于BM-MSC的多种骨再生方法和BM-MSCs的安全性提供了有用的信息。
Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs.