Expression of cell surface Lewis X and Y antigens and FUT4 mRNA is increased in Jurkat cells undergoing apoptosis
Expression of cell surface Lewis X and Y antigens and FUT4 mRNA is increased in Jurkat cells undergoing apoptosis
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DOI：
10.1016/j.bbagen.2004.03.006
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发表时间：
2004-06-11
影响因子：
3
通讯作者：
Matsumoto, K
中科院分区：
文献类型：
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作者：
Azuma, Y;Ito, M;Matsumoto, K
细胞凋亡过程中细胞表面分子发生特异性变化，包括磷脂酰丝氨酸（PS）和某些蛋白质的表达以及糖链的改变。在细胞表面的各种糖链中，刘易斯X（Le（X））和刘易斯Y（Le（Y））抗原是多种生物学过程的关键决定因素。流式细胞仪检测显示，抗Fas抗体诱导的细胞凋亡过程中，细胞表面Le（X）和Le（Y）抗原表达增强。为了阐明Le（x）和Le（Y）表达增强的机制，我们评估了岩藻糖基转移酶（FUT 1、2、3-5-6、4和9）mRNA的表达水平，所述岩藻糖基转移酶主要在Jurkat细胞中表达并且被认为形成Le（X）和Le（Y）。抗Fas抗体作用后FUT 4 mRNA表达上调。此外，Le（X）和Le（Y）抗原水平的增加被半胱天冬酶3或8抑制剂显著抑制。这些结果表明，FUT（主要是FUT 4）的诱导增加了凋亡细胞中Le（X）和Le（Y）的表达，FUT的基因表达由半胱天冬酶3下游的信号介导。(C)2004 Elsevier B. V.保留所有权利。
Cell surface molecules undergo specific changes during apoptosis, including the expression of phosphatidylserine (PS) and some proteins and alterations in sugar chains. Among the various sugar chains on the cell surface, Lewis X (Le(X)) and Lewis Y (Le(Y)) antigens are key determinants for a variety of biological processes.We studied the changes in Le(X) and Le(Y) expression in Jurkat cells, a human T cell line, during apoptosis. Flow cytometry showed that Le(X) and Le(Y) antigen expression was enhanced on the cell surface during apoptosis induced by anti-Fas antibody. To clarify the mechanism of enhanced Le(x) and Le(Y) expression, we assessed the expression levels of fucosyltransferase (FUT1, 2, 3-5-6, 4, and 9) mRNAs that are predominantly expressed in Jurkat cells and which are considered to form Le(X) and Le(Y). The expression of FUT4 mRNA was up-regulated after exposing cells to anti-Fas antibody. Moreover, the increase in Le(X) and Le(Y) antigen levels was significantly suppressed by caspase 3 or 8 inhibitors. These results indicated that the induction of FUT (mainly FUT4), the gene expression of which is mediated by signals downstream of caspase 3, increases Le(X) and Le(Y) expression in apoptotic cells. (C) 2004 Elsevier B.V. All rights reserved.