RELATIONSHIP BETWEEN X-RAY-EXPOSURE AND MALIGNANT TRANSFORMATION IN C3H 10T1/2 CELLS
RELATIONSHIP BETWEEN X-RAY-EXPOSURE AND MALIGNANT TRANSFORMATION IN C3H 10T1/2 CELLS
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DOI：
10.1073/pnas.77.12.7262
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
LITTLE, JB
中科院分区：
文献类型：
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作者：
KENNEDY, AR;FOX, M;LITTLE, JB
小鼠细胞的C3 H 10 T1/2系在X射线照射后出现转化灶需要广泛的增殖，然后在汇合条件下长时间孵育。当将辐照细胞的后代重悬并铺板以确定潜在转化灶的数量时，绝对产率在宽范围的稀释度内是恒定的，并且与未重悬的培养物中观察到的结果相似。对于暴露于给定X射线剂量的细胞，每个培养皿的转化灶的数量与照射细胞的数量无关。显然，很少（如果有的话）的转化克隆发生的直接后果的X射线曝光，挑战的假设，即转化灶是克隆产品的偶尔细胞经历了X射线诱导的突变变化。这至少涉及两个步骤。暴露于X射线可能导致许多或所有细胞发生变化，例如诱导或表达某些细胞功能，并且这种变化可能传递给存活细胞的后代;这种变化的结果是当这些细胞保持在融合条件下时，发生第二步转化的概率增加。
The appearance of transformed foci after x-irradiation of the C3H 10T1/2 line of murine cells requires extensive proliferation followed by prolonged incubation under conditions of confluence. When the progeny of irradiated cells are resuspended and plated to determine the number of potential transformed foci, the absolute yield is constant over a wide range of dilutions and is similar to that observed in cultures that have not been resuspended. For cells exposed to a given X-ray dose, the number of transformed foci per dish is independent of the number of irradiated cells. Apparently, few, if any, of the transformed clones occur as a direct consequence of the X-ray exposure, challenging the hypothesis that transformed foci are the clonal products of occasional cells that have experienced an X-ray-induced mutational change. It appears that at least 2 steps are involved. Exposure to X-rays may result in a change, for example, the induction or expression of some cell function, in many or all of the cells and this change may be transmitted to the progeny of the surviving cells; a consequence of this change would be an enhanced probability of the occurrence of a 2nd step, transformation, when these cells are maintained under conditions of confluence.