INFLUENCE OF CROSS-CORRELATION BETWEEN DIPOLAR AND ANISOTROPIC CHEMICAL-SHIFT RELAXATION MECHANISMS UPON LONGITUDINAL RELAXATION RATES OF N-15 IN MACROMOLECULES
INFLUENCE OF CROSS-CORRELATION BETWEEN DIPOLAR AND ANISOTROPIC CHEMICAL-SHIFT RELAXATION MECHANISMS UPON LONGITUDINAL RELAXATION RATES OF N-15 IN MACROMOLECULES
复制标题
DOI：
10.1016/0009-2614(90)85567-v
复制
发表时间：
1990-12-21
影响因子：
2.8
通讯作者：
CAMPBELL, ID
中科院分区：
文献类型：
--
作者：
BOYD, J;HOMMEL, U;CAMPBELL, ID
肽键 N-15 核的分子内偶极-偶极和化学位移各向异性弛豫机制具有相当的幅度。 使用蛋白质人表皮生长因子 (hEGF) 分析这两种松弛机制之间的互相关（或干扰）。 结果表明，纵向 N-15 弛豫恢复不是由唯一的时间常数定义的。 讨论了专门监测两种弛豫机制之间互相关性的二维实验。 它还表明，包含 H-1 宽带去耦的简单程序消除了互相关对 N-15 纵向弛豫的影响，并导致指数 N-15 恢复。
The intramolecular dipole-dipole and chemical shift anisotropy relaxation mechanisms for the N-15 nucleus of the peptide bond have comparable magnitudes. The cross-correlation (or interference) between these two relaxation mechanisms is analysed using the protein human epidermal growth factor (hEGF). It is demonstrated that the longitudinal N-15 relaxation recovery is not defined by a unique time constant. A two-dimensional experiment to monitor specifically the cross-correlation between the two relaxation mechanisms is discussed. It is also shown that a simple procedure, the inclusion of H-1 broadband decoupling, removes the effects of the cross-correlation upon the N-15 longitudinal relaxation and leads to exponential N-15 recovery.