Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population.
Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population.
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DOI：
10.1534/g3.115.021584
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发表时间：
2015-09-28
期刊：
影响因子：
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通讯作者：
Mackay IJ
中科院分区：
文献类型：
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作者：
Cockram J;Scuderi A;Barber T;Furuki E;Gardner KA;Gosman N;Kowalczyk R;Phan HP;Rose GA;Tan KC;Oliver RP;Mackay IJ
坏死性真菌nodorum Parastagonospora nodorum是世界上最重要的经济谷物作物之一小麦（Triticum aestivum L.）的重要病原体。结核分枝杆菌产生坏死性蛋白效应物，介导宿主细胞死亡，为感染过程的继续提供营养。最近发现的病原体效应物已经彻底改变了作物物种坏死性疾病的抗病育种，使通常复杂的遗传抗性机制被分解成组成部分。到目前为止，在芽孢杆菌中已经确定了三种效应器。在这里，我们使用效应子SnTox1筛选了来自8亲本多亲本高级代杂交（即MAGIC）群体的642个后代，并使用90,000个特征的单核苷酸多态性阵列进行基因分型。MAGIC创始人对SnTox1表现出一定的敏感性，在后代中有明显的海侵分离。SnTox1敏感性表现出较高的遗传力，数量性状位点分析将Snn1位点精确定位在1B染色体短臂上。此外，在5A染色体长臂上发现了一个先前未描述的SnTox1敏感位点，本文命名为QSnn.niab-5A.1。将Snn1位点的单核苷酸多态性峰值转化为KASP基因分型平台，为育种者和研究人员提供了一种简单、廉价的Snn1等位基因状态诊断标记。
The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.