RAT CORTICAL-NEURONS IN CELL-CULTURE - CULTURE METHODS, CELL MORPHOLOGY, ELECTROPHYSIOLOGY, AND SYNAPSE FORMATION
RAT CORTICAL-NEURONS IN CELL-CULTURE - CULTURE METHODS, CELL MORPHOLOGY, ELECTROPHYSIOLOGY, AND SYNAPSE FORMATION
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DOI：
10.1016/0006-8993(78)90476-6
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
DICHTER, MA
中科院分区：
文献类型：
--
作者：
DICHTER, MA
取胚胎15天的大鼠皮质神经元，在分离的细胞培养中培养，体外培养8-12周。神经元在原位发育成类似于成熟皮质神经元的形态，经Ag染色，表现出与皮质神经元相似的被动和主动电生理特性。广泛的化学兴奋性和抑制性突触从头发展。这些培养物可为哺乳动物中枢神经系统神经生理学、递质药理学、病理生理学和药物作用机制的研究提供模型。
Rat cortical neurons from 15 day embryos are grown in dissociated cell culture and maintained in vitro for 8-12 wk. The neurons develop into forms which resemble mature cortical neurons in situ, stain with Ag and exhibit passive and active electrophysiological properties similar to those of cortical neurons. Extensive chemical excitatory and inhibitory synapses develop de novo. These cultures can provide a model for studies of mammalian CNS neuronal physiology, transmitter pharmacology, pathophysiology and mechanisms of drug action.