Juvenile hormone-receptor complex acts on mcm4 and mcm7 to promote polyploidy and vitellogenesis in the migratory locust.
Juvenile hormone-receptor complex acts on mcm4 and mcm7 to promote polyploidy and vitellogenesis in the migratory locust.
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保幼激素受体复合物作用于Mcm4和Mcm7促进飞蝗多倍体和卵黄发生
DOI：
10.1371/journal.pgen.1004702
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发表时间：
2014-10
期刊：
影响因子：
4.5
通讯作者：
Zhou S
中科院分区：
文献类型：
--
作者：
Guo W;Wu Z;Song J;Jiang F;Wang Z;Deng S;Walker VK;Zhou S
保幼激素（JH）是昆虫咽侧体产生的一种倍半萜类化合物，对昆虫的生长、变态和繁殖起着重要的调节作用。虽然JH抑制幼虫变态的作用已经得到了很好的研究，但JH促进成虫繁殖的分子基础尚未完全阐明。甲氧普仑耐受（Met），JH受体，最近已被证明介导的JH行动在变态以及在卵黄发生，但再次，后者的确切机制一直缺乏。我们现在已经证明了使用Met RNAi表型复制一个JH剥夺条件下的飞蝗，JH刺激DNA复制和增加倍性，准备卵黄发生。DNA复制途径中的两个基因Mcm 4和Mcm 7在JH和Met存在下表达。Mcm 4或Mcm 7的耗竭抑制DNA的从头合成和多倍体化，并导致卵黄蛋白原mRNA水平的大幅下降，以及严重受损的卵母细胞成熟和卵巢生长。通过使用荧光素酶报告和电泳迁移率变动分析，我们已经表明，Met直接调节Mcm 4和Mcm 7的转录通过结合到上游的共识序列与E盒或E盒样基序。我们的工作表明，JH-受体复合物作用于Mcm 4和Mcm 7，以调节DNA复制和多倍体卵黄发生和卵母细胞成熟。
Juvenile hormone (JH), a sesquiterpenoid produced by the corpora allata, coordinates insect growth, metamorphosis, and reproduction. While JH action for the repression of larval metamorphosis has been well studied, the molecular basis of JH in promoting adult reproduction has not been fully elucidated. Methoprene-tolerant (Met), the JH receptor, has been recently shown to mediate JH action during metamorphosis as well as in vitellogenesis, but again, the precise mechanism underlying the latter has been lacking. We have now demonstrated using Met RNAi to phenocopy a JH-deprived condition in migratory locusts, that JH stimulates DNA replication and increases ploidy in preparation for vitellogenesis. Mcm4 and Mcm7, two genes in the DNA replication pathway were expressed in the presence of JH and Met. Depletion of Mcm4 or Mcm7 inhibited de novo DNA synthesis and polyploidization, and resulted in the substantial reduction of vitellogenin mRNA levels as well as severely impaired oocyte maturation and ovarian growth. By using luciferase reporter and electrophoretic mobility shift assays, we have shown that Met directly regulates the transcription of Mcm4 and Mcm7 by binding to upstream consensus sequences with E-box or E-box-like motifs. Our work suggests that the JH-receptor complex acts on Mcm4 and Mcm7 to regulate DNA replication and polyploidy for vitellogenesis and oocyte maturation.
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影响因子：
4.8
作者：
Bernardo, Travis J.;Dubrovsky, Edward B.
通讯作者：
Dubrovsky, Edward B.
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
DOI：
10.1073/pnas.030539597
发表时间：
2000-02-15
影响因子：
11.1
作者：
Chong, JPJ;Hayashi, MK;Stillman, B
通讯作者：
Stillman, B
影响因子：
4.8
作者：
Kanter, Diane M.;Bruck, Irina;Kaplan, Daniel L.
通讯作者：
Kaplan, Daniel L.
影响因子：
3.8
作者：
Glinka, AV;Wyatt, GR
通讯作者：
Wyatt, GR