15N2 fixation, H2 evolution and C2H2 reduction relationships in Phaseolus vulgaris
15N2 fixation, H2 evolution and C2H2 reduction relationships in Phaseolus vulgaris
复制标题
菜豆中 15N2 固定、H2 演化和 C2H2 还原关系
DOI：
10.1111/j.1399-3054.1980.tb08643.x
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发表时间：
1980
影响因子：
6.4
通讯作者：
E. Salati
中科院分区：
文献类型：
--
作者：
S. Saito;E. Matsui;E. Salati
在一项豆类 (Phaseolus vulgaris L.) 根瘤中 N2 固定的研究中。不同年龄的Venezuela-350在受控的光照和温度条件下保存，测量了三个参数——15N2固定、C2H2还原和H2释放。
C2H2还原与N固定呈正相关。采样标准化对于将实验误差保持在最低限度是必要的。为了减少重复之间的差异，应仔细孵育结节（足够的时间和温度），并且还应仔细保存用于色谱分析的样品。在测量的第一个小时内，所研究的系统（结节根、受干扰的整株植物和完整植物）中的 C2H2 还原没有发现显着差异。
在 20 天以上的根瘤中检测到 H2 演化，并且与 N2 固定和 C2H2 减少呈正相关。该测量对于解释电子平衡比中三个参数之间的平衡至关重要。在植物温室条件下，最大的氢气释放发生在种植后 20-30 天。
使用不同植物的结节根来确定 N2 固定和 C2H2 减少量，平均而言，所有年龄段的 C2H2:N2 比率在 7.3 和 8.3 之间变化。体内C2H4/(3N2+H2)电子平衡值超过1，变化范围为1.32至1.43。这些数据表明，即使考虑了 H2 的演化，在使用 C2H2 还原技术时也可能会高估实际的 N2 固定。在这种情况下，应谨慎使用此方法，以避免高估实际值平均 32-43%。当使用较年轻的结节时，三个参数的测量之间的变化较小。
In a study of N2 fixation in nodules of beans (Phaseolus vulgaris L.) cv. Venezuela-350 of different ages and kept under controlled light and temperature conditions, three parameters - 15N2 fixation, C2H2 reduction and H2 evolution—were measured.
C2H2 reduction was positively related to the N fixed. A standardization of sampling is necessary to keep experimental errors to a minimum. To decrease the variation between replications, the nodules should be carefully incubated (adequate time and temperature), and the samples for chromatography should also be carefully stored. No significant difference was found in C2H2 reduction in the systems studied - nodulated roots, disturbed whole plants and intact plants—during the first hour of measurement.
H2 evolution was detected in nodules over 20 days of age and was positively related to N2 fixation and C2H2 reduction. This measurement was essential to account for the equilibrium between the three parameters in the electron balance ratio. Under phytotron conditions, maximum H2 evolution occurred 20–30 days after planting.
Nodulated roots from different plants were used to determine the N2 fixed and C2H2 reduction, and on average the ratios C2H2:N2 varied between 7.3 and 8.3 for all ages. In vivo C2H4/(3N2+ H2) electron balance values were over 1, varying from 1.32 to 1.43. These data suggest that overestimation of actual N2 fixation might be occurring when using the C2H2 reduction technique, even if taking account for H2 evolution. In this case, this method should be used with great care to avoid an overestimation of on average 32-43% of the actual value. Variation was smaller between the measures of the three parameters when younger nodules were used.