Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea
Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea
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DOI：
10.1046/j.1439-0434.2003.00697.x
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发表时间：
2003-03-01
期刊：
影响因子：
--
通讯作者：
Urbanek, H
中科院分区：
文献类型：
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作者：
Patykowski, J;Urbanek, H
测定了两个对灰霉病菌感病性不同的番茄品种的未感病和感病叶片的过氧化氢产生速率。分析了超氧阴离子、羟自由基、抗坏血酸含量以及超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、还原型谷胱甘肽过氧化物酶（NADH）活性的变化。受感染的叶子过氧化氢水平增加。它是更大的，一般发生在较不敏感的品种植物。Perkoz比那些更敏感的cv。科林多硝基四氮唑蓝的诱导使质外体中的还原活性和SOD含量较高。Perkoz接种后24 h。在对照中，感病品种质外体中的NADH过氧化物酶活性较高。Corindo，但在感染后，它增加得更快，并在不太敏感的CV更高的水平。Perkoz NADH氧化被抑制只有15%的特异性抑制剂DPI（二苯基碘），但完全被KCN和NaN3抑制。APX活性增加类似48小时后，过氧化氢酶活性的小幅度增加，观察到在两个品种感染后不久。这些结果表明番茄植株对坏死营养型真菌B的感染的抗性。灰霉病的发生可能是由于NADH过氧化物酶和SOD在质外体空间早期刺激过氧化氢和超氧自由基的产生，证实了它们在植物质外体空间中的增强产生的重要作用。
Hydrogen peroxide generation rates of uninfected and infected leaves of two tomato (Lycopersicon esculentum) cultivars showing differential susceptibility to Botrytis cinerea were determined. The superoxide anion, hydroxyl radical, ascorbate contents and changes in NADH peroxidase, superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities in the apoplast fraction were analysed. Infected leaves had an increased hydrogen peroxide level. It was greater and generally occurred earlier in plants of the less susceptible cv. Perkoz than in those of the more susceptible cv. Corindo. Induction of nitrotetrazolium blue reducing activity and SOD levels in apoplast were higher in cv. Perkoz 24 h after inoculation. In the controls, NADH peroxidase activity in apoplast was higher in the more susceptible cv. Corindo, but after infection it increased faster and to a higher level in the less susceptible cv. Perkoz. NADH oxidation was inhibited by only 15% by a specific inhibitor DPI (diphenylene-iodonium) but was completely inhibited by KCN and NaN3. Similar increases in APX activity after 48 h and a small increase in catalase activities were observed in both cultivars soon after infection. These results indicate that resistance of tomato plants to infection by the necrotrophic fungus B. cinerea may result from early stimulation of hydrogen peroxide and superoxide radical generations by NADH peroxidase and SOD in apoplastic space, and they confirm the important role of their enhanced production in apoplastic spaces of plants.