Cholinergic, nitrergic and peptidergic (substance P- and CGRP-utilizing) innervation of the horse intestine. A histochemical and immunohistochemical study
Cholinergic, nitrergic and peptidergic (substance P- and CGRP-utilizing) innervation of the horse intestine. A histochemical and immunohistochemical study
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DOI：
10.14670/hh-19.357
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发表时间：
2004-04-01
影响因子：
2
通讯作者：
Dolera, M
中科院分区：
文献类型：
--
作者：
Domeneghini, C;Radaelli, G;Dolera, M
对成年马的小肠和大肠进行了组织化学和免疫化学研究，以证明肠壁内神经系统的组成部分。通过使用Nissl-硫堇染色以及抗神经丝200（NF 200）免疫反应检查了壁内神经系统的一般结构组织，这表明粘膜下以及肌间神经丛中存在神经元。在大肠中还发现了浆膜下神经节。乙酰胆碱酯酶（AChEase）的活性，观察粘膜下和肌间神经丛。胆碱乙酰转移酶（ChAT）和囊泡乙酰胆碱转运蛋白（VAChT）的免疫组织化学反应也证明了乙酰胆碱利用神经元的本地化。与组织化学和免疫组织化学可能胆碱能神经纤维检测到的内部肌肉组织。两种可能的胆碱能共介质降钙素基因相关肽（CGRP）和P物质（SP）已被研究的免疫组织化学方法。CGRP的免疫反应，以及在圆形的神经细胞体中的粘膜下神经丛的神经纤维中检测到，而SP的免疫反应证明在神经纤维的图尼卡粘膜，在神经细胞体和纤维的粘膜下神经丛和肌间神经丛的神经纤维。NADPH-黄递酶的反应，这是连接到一氧化氮的合成和释放，在神经细胞体和神经纤维的粘膜下和肌间神经丛，以及在一个subserovial本地化的大肠检测。通过抗一氧化氮合酶（NOS）免疫反应证实了其氮能成分。结果进行了比较与其他哺乳动物和相关的复杂的肠道马的生理和病理生理。
The small and large intestine of adult horses were histochemically and immunohistochemically investigated in order to evidence components of the intramural nervous system. The general structural organization of the intramural nervous system was examined by using Nissl-thionin staining as well as the anti-neurofilament 200 (NF200) immunoreaction, which demonstrated the presence of neurons in the submucous as well as myenteric plexuses. The additional presence of subserosal ganglia was shown in the large intestine. Acetylcholinesterase (AChEase) activity was observed in both the submucous and myenteric plexuses. Localization of acetylcholine-utilizing neurons was also evidenced by immunohistochemical reactions for choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT). With both histochemistry and immunohistochemistry possible cholinergic nerve fibres were detected in the inner musculature. The two possible cholinergic co-mediators Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) have been investigated by an immunohistochemical approach. CGRP immunoreactivity was detected in roundish nerve cell bodies as well as in nerve fibres of the submucous plexus, whereas SP immunoreactivity was evidenced in nerve fibres of the tunica mucosa, in nerve cell bodies and fibres of the submucous plexus and in nerve fibres of the myenteric plexus. NADPH-diaphorase reactivity, which is linked to the synthesis and release of nitric oxide, was detected in nerve cell bodies and nerve fibres of both the submucous and myenteric plexuses as well as in a subserosal localization of the large intestine. The nitrergic components were confirmed by the anti-NOS (nitric oxide synthase) immunoreaction. Results are compared with those of other mammals and related to the complex intestinal horse physiology and pathophysiology.