Alginate/gelatin encapsulation promotes NK cells differentiation potential of bone marrow resident C-kit+ hematopoietic stem cells
Alginate/gelatin encapsulation promotes NK cells differentiation potential of bone marrow resident C-kit+ hematopoietic stem cells
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DOI：
10.1016/j.ijbiomac.2021.02.131
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发表时间：
2021-02-24
影响因子：
8.2
通讯作者：
Valipour, Behnaz
中科院分区：
文献类型：
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作者：
Fathi, Ezzatollah;Farahzadi, Raheleh;Valipour, Behnaz
自然杀伤(NK)细胞在不需要事先致敏的情况下摧毁癌细胞的能力使它们成为细胞治疗的候选细胞。在细胞分化过程中，水凝胶的应用必须作为细胞输送载体予以通知。本研究探讨海藻酸盐-明胶微球对C-KIT(+)细胞NK细胞分化潜能的影响。C-kit(+)细胞在包膜和非包膜条件下均可分化为NK细胞。接下来，对细胞进行实时聚合酶链式反应(PCR)和Western blotting，以分别评估它们的端粒长度和蛋白质表达。收集培养上清液，测定细胞因子水平。分化后的NK细胞与Molt-4细胞共培养，Annexin V/PI法检测细胞凋亡率。Janus激酶/信号转导(JAK/STAT)通路组件的蛋白表达发生了显著变化。此外，包埋剂还能增加Molt-4细胞的凋亡率和NK细胞分化为C-kit(+)细胞的端粒长度。因此，包埋对NK细胞向C-kit(+)细胞分化的影响可能与IL-2、IL-3、IL-7、IL-12等细胞因子的分泌以及端粒长度的增加有关。(C)2021年爱思唯尔B.V.保留所有权利。
The ability of natural killer (NK) cells to destroy cancerous cells with no prior sensitization has made them attractive candidates for cell therapy. The application of hydrogels must be notified as cell delivery vehicles in cell differentiation. The present study was conducted to investigate the effect of alginate-gelatin encapsulation on NK cell differentiation potential of C-kit(+) cells. C-kit(+) cells were differentiated to NK cells under both encapsulated and un-encapsulated conditions. Next, the cells were subjected to real-time polymerase chain reaction (PCR) and western blotting for the assessment of their telomere length and protein expressions, respectively. Afterward, culture medium was collected to measure cytokines levels. Thereafter, the differentiated NK cells were co cultured with Molt-4 cells to investigate the potency of cell apoptosis by Annexin V/PI assay. A significant change was observed in the protein expression of Janus kinase/Signal transducers (JAK/STAT) pathway components. Additionally, the encapsulation caused an increase in the apoptosis of Molt-4 cells and telomere length of NK cells differentiated C-kit(+) cells. Therefore, it can be concluded that the effects of encapsulation on NK cell's differentiation of C-kit(+) cells could be resulted from the secreted cytokines of interleukin (IL)-2, IL-3, IL-7, and IL-12 as well as the increased telomere length. (c) 2021 Elsevier B.V. All rights reserved.