Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC)
Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC)
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DOI：
10.1093/emboj/19.12.3049
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发表时间：
2000-06-15
期刊：
影响因子：
11.4
通讯作者：
Becker, PB
中科院分区：
文献类型：
--
作者：
Corona, DFV;Eberharter, A;Becker, PB
果蝇的ISWI ATP酶是一种分子引擎，可以在体外驱动一系列核小体重塑反应。ISWI对于细胞活力、发育基因表达和染色体结构是重要的。它与其他蛋白质相互作用，形成几个不同的核小体重塑机器。染色质可及性复合物（CHRAC）是一种含有ISWI和其他几种蛋白质的生物化学实体。在这里，我们报告的两个最小的CHRAC亚基，CHRAC-14和CHRAC-16的鉴定。它们含有分别与序列特异性转录因子NF-YB和NF-YC中发现的组蛋白折叠结构域最密切相关的组蛋白折叠结构域，CHRAC-14和CHRAC-16彼此直接相互作用以及与ISWI直接相互作用，并且与功能活性CHRAC相关。这两个亚基的发育表达谱表明，两个组蛋白折叠亚基在早期胚胎的染色质重塑反应中具有专门的作用。
The ISWI ATPase of Drosophila is a molecular engine that can drive a range of nucleosome remodelling reactions ire vitro. ISWI is important for cell viability, developmental gene expression and chromosome structure. It interacts with other proteins to form several distinct nucleosome remodelling machines. The chromatin accessibility complex (CHRAC) is a biochemical entity containing ISWI in association with several other proteins. Here we report on the identification of the two smallest CHRAC subunits, CHRAC-14 and CHRAC-16. They contain histone fold domains most closely related to those found in sequence-specific transcription factors NF-YB and NF-YC, respectively, CHRAC-14 and CHRAC-16 interact directly with each other as well as with ISWI, and are associated with functionally active CHRAC. The developmental expression profiles of both subunits suggest specialized roles in chromatin remodelling reactions in the early embryo for both histone fold subunits.