GAMMA-INTERFERON TREATMENT INHIBITS COLLAGEN DEPOSITION IN MURINE SCHISTOSOMIASIS
GAMMA-INTERFERON TREATMENT INHIBITS COLLAGEN DEPOSITION IN MURINE SCHISTOSOMIASIS
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DOI：
10.1002/hep.1840100508
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发表时间：
1989-11-01
期刊：
影响因子：
13.5
通讯作者：
ZERN, MA
中科院分区：
文献类型：
--
作者：
CZAJA, MJ;WEINER, FR;ZERN, MA
由于干扰素已显示在几种体外系统中影响基质蛋白如胶原蛋白的合成，因此γ-干扰素的潜在作用是：观察干扰素对肝纤维化的抑制作用。主要由肝细胞组成的肝细胞用重组γ-北方印迹杂交显示γ-干扰素干扰素治疗引起前-α 2（I）胶原mRNA水平的显著降低，但β-肌动蛋白mRNA含量。γ的影响-然后在肝纤维化的体内模型，鼠血吸虫病中研究干扰素。血吸虫感染的小鼠每天i.m.注射γ-在初始感染后4周开始，持续4周干扰素。γ-干扰素治疗减少胶原沉积所确定的组织学评价和总肝胶原含量的测量。北方印迹显示处理的感染动物的I型和III型前胶原mRNA水平仅为感染对照的32%和29%，但β-胶原mRNA水平为感染对照的32%和29%。肌动蛋白mRNA水平显著升高。这些结果表明γ的潜在作用。干扰素作为体内抗纤维化剂。
Since interferons have been shown to affect the synthesis of matrix proteins such as collagen in several in vitro system, the potential role of .gamma.-interferon in inhibiting hepatic fibrosis was investigated. Hepatic cells, consisting primarily of hepatocytes, were treated with recombinant .gamma.-interferon for 24 hr. Northern blot hybridization showed that .gamma.-interferon treatment caused a profound decrease in pro-.alpha.2(I)collagen mRNA levels but an increase in .beta.-actin mRNA content. The effects of .gamma.-interferon were then studied in an in vivo model of hepatic fibrogenesis, murine schistosomiasis. Schistosoma-infected mice were treated with daily i.m. injections of .gamma.-interferon for a 4-week period starting 4 weeks after the initial infection. .gamma.-Interferon treatment decreased collagen deposition as determined by histologic evaluation and measurement of total liver collagen content. Northern blots showed Types I and III procollagen mRNA levels for treated, infected animals to be only 32 and 29% that of infected controls, but .beta.-actin mRNA levels were significantly elevated. These results indicate a potential role for .gamma.-interferon as an antifibrogenic agent in vivo.