Nuclear activity from F9 embryonal carcinoma cells binding specifically to the enhancers of wild-type polyoma virus and PyEC mutant DNAs.
Nuclear activity from F9 embryonal carcinoma cells binding specifically to the enhancers of wild-type polyoma virus and PyEC mutant DNAs.
复制标题
F9 胚胎癌细胞的核活性与野生型多瘤病毒和 PyEC 突变 DNA 的增强子特异性结合。
DOI：
10.1093/nar/14.7.2845
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
Fujimura,FK
中科院分区：
文献类型：
--
作者：
Fujimura,FK
尽管野生型多瘤病毒不能有效地感染小鼠胚胎癌（EC）细胞，但已经分离出许多能够感染未分化EC细胞的突变体（PyEC突变体）。所有 PyEC 突变体在病毒基因组的增强子区域内都有 DNA 序列改变。该报告描述了 F9 EC 细胞核提取物中存在的一种活性，通过“足迹”分析，该活性与多瘤增强子区域（指定为 B 或 6 元件）内约 20 个碱基对（核苷酸 5180-5200）的小区域特异性结合。虽然在野生型多瘤病毒增强子和PyEC突变体的增强子、PyFll和PyF441之间没有检测到因子的结合差异，PyFll和PyF441已被选择用于F9细胞的有效感染，但在用DNA酶I或核酸外切酶III消化它们的裸露DNA的模式中观察到野生型和突变体之间的明确差异。这种差异仅限于这些突变 DNA 共有的点突变（核苷酸 5258）周围的区域。
Although wild-type polyoma virus does not productively infect murine embryonal carcinoma (EC) cells, a number of mutants (PyEC mutants) that do infect undifferentiated EC cells have been isolated. All PyEC mutants have DNA sequence alterations within the enhancer region of the viral genome. This report describes an activity present in nuclear extracts of F9 EC cells which, by “footprint” analyses, binds specifically to a small region of about 20 base pairs (nucleotides 5180–5200) within the subregion of the polyoma enhancer designated as the B or 6 element. While no difference in binding of factor was detected between wild-type polyoma enhancer and the enhancers of the PyEC mutants, PyFlll and PyF441, which had been selected for productive infection of F9 cells, definite differences between wild-type and mutants were observed in the digestion patterns of their naked DNAs with either DNAase I or exonuclease III. This difference was restricted to the region around the point mutation (nucleotide 5258) common to these mutant DNAs.