Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines
Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines
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DOI：
10.1007/s00418-019-01842-z
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发表时间：
2020-04-01
影响因子：
2.3
通讯作者：
Kataoka, Takao
中科院分区：
文献类型：
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作者：
Baba, Kosuke;Kuwada, Sara;Kataoka, Takao
溶酶体相关膜蛋白1（Lysosomal-associated membrane protein 1，LAMP 1）主要定位于溶酶体和晚期内体。我们在此研究了5种哺乳动物培养细胞系中溶酶体膜蛋白的细胞内定位。与增强型绿色荧光蛋白（EGFP）融合的大鼠LAMP 1主要积聚在特定的细胞质区域，并且在金黄仓鼠肾BHK-21细胞和中国仓鼠卵巢CHO-K1细胞中几乎不与LysoTracker（R）Red DND-99共定位。金黄地鼠、中国仓鼠和人的LAMP 1-EGFP在BHK-21细胞中显示出与大鼠LAMP 1-EGFP相似的细胞内分布。在BHK-21细胞和CHO-K1细胞的核周区也检测到内源性LAMP 1，并在BHK-21细胞中与大鼠CD 63-EGFP共定位。此外，大鼠LAMP 1-DsRed-Mp53与人trans-Golgi网络蛋白2-EGFP在BHK-21细胞中共定位良好。这些结果表明，在BHK-21细胞中，LAMP 1主要定位于trans-Golgi网络。
Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulated at a particular cytoplasmic area and barely co-localized with LysoTracker (R) Red DND-99 in golden hamster kidney BHK-21 cells and Chinese hamster ovary CHO-K1 cells. Golden hamster, Chinese hamster, and human LAMP1-EGFP showed a similar intracellular distribution to rat LAMP1-EGFP in BHK-21 cells. Endogenous LAMP1 was also detected in a perinuclear area in BHK-21 cells and CHO-K1 cells, and co-localized with rat CD63-EGFP in BHK-21 cells. Moreover, rat LAMP1-DsRed-Monomer co-localized well with the human trans-Golgi network protein 2-EGFP in BHK-21 cells. These results reveal that LAMP1 predominantly localizes to the trans-Golgi network in BHK-21 cells.