Isolation and expression analysis of two novel C-repeat binding factor (CBF) genes involved in plant growth and abiotic stress response in mangrove Kandelia obovata
Isolation and expression analysis of two novel C-repeat binding factor (CBF) genes involved in plant growth and abiotic stress response in mangrove Kandelia obovata
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红树秋茄植物生长和非生物胁迫反应中两个新的C重复结合因子（CBF）基因的分离和表达分析
DOI：
10.1007/s10646-020-02219-y
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发表时间：
2020-05-12
期刊：
影响因子：
2.7
通讯作者：
Sun, Cui-Ci
中科院分区：
文献类型：
--
作者：
Peng, Ya-Lan;Wang, You-Shao;Sun, Cui-Ci
红树是中国沿海的耐寒红树植物之一。目的：揭示k的耐寒机理。本研究从冷胁迫的k中分离到了两个ocbf /DREB1基因（命名为kocbf1和kocbf3）。研究了它们在不同器官和多种非生物胁迫下的表达谱。kocbf1和kocbf3的蛋白均含有cbf的特异性特征，分别与avicennia marina的amcbf1和amcb3具有较高的相似性。在不同的非生物胁迫和外源激素作用下，两种ocb4的表达模式不同，说明它们可能具有不同的调控机制，参与不同的调控途径。这两个基因在基础和低温诱导下的高表达表明它们可能在植物生长和抗寒中发挥重要作用。盐和铅（Pb2+）处理对kocbf3的显著诱导表明，该基因也可能参与对盐和重金属胁迫的响应。本研究将为红树生物技术育种、高纬度移栽和重金属污染的生物修复提供理论依据。
Kandelia obovatais one of the cold tolerant mangrove plants along the China coast. To reveal the cold tolerant mechanism ofK. obovata, the present work isolated twoCBF/DREB1 genes (designatedKoCBF1 andKoCBF3) from cold-stressedK. obovataand characterized their expression profiles in various organs and in response to multiple abiotic stresses. The deduced proteins ofKoCBF1 and 3 all contain specific features of CBFs, and show high similarity toAmCBF1 and 3 fromAvicennia marina, respectively. Different expression patterns of the twoCBForthologous under various abiotic stresses and exogenous hormone suggested that they may have different regulators and be involved in different regulatory pathway. The high basal and cold induced expression of the two genes indicated that they may all play important roles in growth and cold resistance of plants. The significant induction ofKoCBF3 after salt and lead (Pb2+) treatments suggested that thisCBFgene may also participate in response to salinity and heavy metal stresses. This study will provide a better understanding of CBF-regulated stress-resistant mechanism, which may be benefit in mangrove biotechnological breeding, high-latitude transplanting, and bioremediation of heavy metal pollutions.